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Estimation of polyploidy and aneuploidy by non-radioactive in situ hybridization






Mededelingen van de Faculteit Landbouwwetenschappen Universiteit Gent 57(3 PART B): 987-992

Estimation of polyploidy and aneuploidy by non-radioactive in situ hybridization



Accession: 002373350



Related references

D.S.rio, A.; Vagnarelli, P.; D.C.rli, L., 1990: Aneuploidy assay on diethylstilbestrol by means of in situ hybridization of radioactive and biotinylated DNA probes on interphase nuclei. Aneuploidy tests by means of in situ hybridization with chromosome-specific DNA probes on interphase nuclei have been carried out on human lymphocytes treated with diethylstilbestrol (DES). A DNA probe specific for chromosome Y (Y97), either radio...

Downie, S.E.; Flaherty, S.P.; Swann, N.J.; Matthews, C.D., 1997: Estimation of aneuploidy for chromosomes 3, 7, 16, X and Y in spermatozoa from 10 normospermic men using fluorescence in-situ hybridization. Fluorescence in-situ hybridization (FISH) is a fast and efficient method of estimating aneuploidy in human spermatozoa. In this study, we have estimated baseline disomy frequencies in spermatozoa from a group of 10 normospermic men, using stringen...

Downie, S.E.; Flaherty, S.P.; Matthews, C.D., 1997: Detection of chromosomes and estimation of aneuploidy in human spermatozoa using fluorescence in-situ hybridization. The development and application of fluorescence in-situ hybridization (FISH) has opened the way for comprehensive studies on numerical chromosome abnormalities in human spermatozoa. FISH can be rapidly applied to large numbers of spermatozoa and t...

Morel, F.; Mercier, S.; Roux, C.; Clavequin, M.C.aire; Bresson, J.L.c, 1997: Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization. Objective: To estimate the mean frequency of aneuploidy levels of chromosomes 8, 15, 18, X, and Y in human sperm, while minimizing the effect of individual factors by analyzing sperm samples from a large set of patients. Design: Prospective random...

Morel, F.; Mercier, S.; Roux, C.; Clavequin, M.C.; Bresson, J.L., 1997: Estimation of aneuploidy levels for 8, 15, 18, X and Y chromosomes in 97 human sperm samples using fluorescence in situ hybridization. To estimate the mean frequency of aneuploidy levels of chromosomes 8, 15, 18, X, and Y in human sperm, while minimizing the effect of individual factors by analyzing sperm samples from a large set of patients. Prospective randomized analysis of sp...

Eastmond, D.A.; Pinkel, D., 1990: Detection of aneuploidy and aneuploidy-inducing agents in human lymphocytes using fluorescence in situ hybridization with chromosome-specific DNA probes. The feasibility of utilizing fluorescence in situ hybridization with chromosome-specific DNA probes as the basis of an assay to detect aneuploidy and aneuploidy-inducing agents in interphase human lymphocytes has been investigated. The assay invol...

Pellestor, F., 2006: In situ aneuploidy assessment in human sperm: the use of primed in situ and peptide nucleic acid-fluorescence in situ hybridization techniques. Both the primed in situ (PRINS) and the peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) techniques constitute alternatives to the conventional (fluorescence in situ hybridization, FISH) procedure for chromosomal investigations....

Durakbasi-Dursun, H.Gul.; Zamani, A.Gul.; Kutlu, R.; Görkemli, Hüseyin.; Bahce, M.; Acar, A., 2007: A new approach to chromosomal abnormalities in sperm from patients with oligoasthenoteratozoospermia: detection of double aneuploidy in addition to single aneuploidy and diploidy by five-color fluorescence in situ hybridization using one probe set. Objective: To determine the frequencies of disomy, nullisomy, total aneuploidy, and diploidy in the sperms of infertile men.Design: A controlled prospective study.Setting: Assisted reproductive technology (ART)/IVF Unit and Department of Medical...

Robbins, W.A.; Baulch, J.E.; Moore, D.; Weier, H.U.; Blakey, D.; Wyrobek, A.J., 1995: Three-probe fluorescence in situ hybridization to assess chromosome X, Y, and 8 aneuploidy in sperm of 14 men from two healthy groups: evidence for a paternal age effect on sperm aneuploidy. The method of simultaneous three-chromosome fluorescence in situ hybridization (FISH) was developed using repetitive DNA sequence probes for chromosomes 8, X and Y and applied to semen of 14 men from two healthy groups who differed in their averag...

Hill, D.L., 2003: Aneuploidy screening of preimplantation embryos using comparative genomic hybridization versus fluorescence in situ hybridization techniques. Fertility and Sterility 80(4): 873-4; Discussion 875