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Estimation of riboflavin in food products by riboflavin-binding apoprotein

, : Estimation of riboflavin in food products by riboflavin-binding apoprotein. Voprosy Pitaniya ( 1): 70-72

A rapid, simple method of estimating riboflavin in food hydrolysates based on change in fluorescence (excitation 465 nm, emission 525 nm) after titration with riboflavin-binding apoprotein from egg albumen is described. Comparison with the fluorimetric method of estimating riboflavin after conversion to lumiflavin in alkaline conditions showed good agreement for milk products.

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Related references

Kodentsova, V.M.; Orlova, N.V.; Vrzhesinskaia, O.A.; Sokol'nikov, A.A., 1992: Determining the level of riboflavin in food products by using riboflavin-binding apoprotein. Voprosy Pitaniia: 70-72

Kodentsova, V.M.; Orlova, N.V.; Vrzhesinskaya, O.A.; Sokol'nikov, A.A., 1992: Determination of riboflavin in food products using riboflavin-binding apoprotein. Voprosy Pitaniya 0(1): 70-72

Moonen C.T.W.; Van Den Berg W.A.M.; Boerjan M.; Muller F., 1984: Carbon 13 and nitrogen 15 nmr study on the interaction between riboflavin and riboflavin binding apoprotein. Riboflavin-binding apoproteins from egg yolk and egg white were reconstituted with 15N- and 13C-enriched riboflavin derivatives. These protein preprations were investigated in the oxidized and 2-electron-reduced states by 15N and 13C NMR technique...

Kodentsova, V.M.; Vrzhesinskaia, O.A.; Risnik, V.V.; Sokol'nikov, A.A.; Spirichev, V.B., 1994: Isolation of riboflavin-binding apoprotein from chicken egg protein and its use for determining riboflavin in biological samples. A riboflavine-binding protein has been isolated from egg white and its properties have been characterized. The apoprotein-employing techniques for riboflavine detection in foods, urine, and blood serum are highly competitive with conventional labo...

Kodentsova, V.M.; Vrzhesinskaya, O.A.; Spirichev, V.B., 1995: Fluorometric riboflavin titration in plasma by riboflavin-binding apoprotein as a method for vitamin B2 status assessment. A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B2 status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluoresce...

Moonen, C.T.W.; Van Den Berg, W.A.M.; Boerjan, M., 1984: Carbon-13 and nitrogen-15 nuclear magnetic resonance study on the interaction between riboflavin and riboflavin-binding apoprotein. Biochemistry (American Chemical Society) 23: 73-8

Pueyo, J.J.; Curley, G.P.; Mayhew, S.G., 1996: Kinetics and thermodynamics of the binding of riboflavin, riboflavin 5'-phosphate and riboflavin 3',5'-bisphosphate by apoflavodoxins. The reactions of excess apoflavodoxin from Desulfovibrio vulgaris, Anabaena variabilis and Azotobacter vinelandii with riboflavin 5'-phosphate (FMN), riboflavin 3',5'-bisphosphate and riboflavin are pseudo-first-order. The rates inc...

Anonymous, 1958: Studies in the quality of plant food I Influence of variety and environment upon the riboflavin content of hulled rice 1Investigations on the estimation of riboflavin and the selection of sample rice

Duyvis, M.G.; Hilhorst, R.; Laane, C.; Evans, D.J.; Schmedding, D.J.M., 2002: Role of riboflavin in beer flavor instability: determination of levels of riboflavin and its origin in beer by fluorometric apoprotein titration. A method for the quantitative determination of riboflavin levels in beer was developed. The method is based on the quenching of riboflavin fluorescence, which occurs when riboflavin binds to the aporiboflavin-binding protein from egg white. The me...

Henry, K.M.; Houston, J.; Kon, S.K., 1940: The estimation of riboflavin. Part 2. The estimation of riboflavin in milk : comparison of fluorimetric and biological tests. II. Confirmation of the suitability of the technique described in part 1 has been obtained by its use for the estimation of riboflavin in 2 samples of spray dried milk and 1 of evaporated milk. Fluorimetric determinations of the riboflavin were al...