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Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique






Aquaculture 109(3-4): 367-373

Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique

Milt was collected individually from rainbow trout (Oncorhynchus mykiss), whitefish (Coregonus clupeaformis) and yellow perch (Perca flavescens) and sperm density measured by three different methods: measuring spermtocrit value, counting sperm using a Neubauer counting chamber, and by measuring absorbance at 505 nm. Sperm concentrations significantly differed between yellow perch (41.58 +- 3.5 times 10-9 ml-1) and the two other species, rainbow trout (11.80 +- 6.19 times 10-9) and whitefish (7.95 +- 2.57 times 10-9). In diluted yellow perch sperm and directly sampled sperm of whitefish and rainbow trout, correlation coefficients were highly significant between spermatocrit, counting, and the sperctrophotometric method of measurement. It is suggested that sperm concentration measured by optical density offers a quick and accurate method of determining sperm concentration.

Accession: 002373413

DOI: 10.1016/0044-8486(93)90175-x

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