EurekaMag.com logo
+ Translate

Estimation of xyloglucan in vegetables by enzyme-linked immunosorbent assay


, : Estimation of xyloglucan in vegetables by enzyme-linked immunosorbent assay. Journal of Nutritional Science and Vitaminology 39(6): 597-606

We prepared the antibody specific against plant xyloglucan and applied it to enzyme-linked immunosorbent assay in order to estimate xyloglucan contents in several vegetables. In this experiment, xyloglucan was detected in the 24% aqueous potassium hydroxide-soluble fractions of tomato, cabbage, lettuce, and eggplant. The xyloglucan contents of these vegetables were estimated to be 1.4 mg, 54 micrograms, 14 micrograms, and 4 micrograms per one hundred grams of their edible portions, respectively, using tamarind xyloglucan as a standard xyloglucan.

(PDF same-day service: $19.90)

Accession: 002373526

PMID: 8006716

DOI: 10.3177/jnsv.39.597

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Sone, Y.; Sato, K.; Misaki, A., 1991: Determination of plant xyloglucan by enzyme-linked immunosorbent assay. Agricultural and Biological Chemistry 55: 55-6

Tandhanand-Banchuin, N.; Vannasaeng, S.; Ploybutr, S.; Sriussadaporn, S., 1993: Comparison of anti-human insulin antibodies detection by commercial enzyme-linked immunosorbent assay kit, displacement enzyme-linked immunosorbent assay and radioimmunoassay, in Thai diabetic patients. Antibodies specific to human insulin, in sera of Thai diabetic patients and normal healthy individuals, were detected by a commercial enzyme-linked immunosorbent assay (ELISA) kit, displacement ELISA and radioimmunoassay (RIA). Among 36 insulin-tr...

Tandhanand Banchuin, N.; Vannasaeng, S.; Ploybutr, S.; Sriussadaporn, S., 1993: Comparison of anti-human insulin antibodies detection by commercial enzyme-linked immunosorbent assay kit, displacement enzyme-linked immunosorbent assay and radioimmunoassay, in Thai diabetic patients. Antibodies specific to human insulin, in sera of Thai diabetic patients and normal healthy individuals, were detected by a commercial enzyme-linked immunosorbent assay (ELISA) kit, displacement ELISA and radioimmunoassay (RIA). Among 36 insulin-tr...

van Loon, A.M.; van der Logt, J.T.; Heessen, F.W.; van der Veen, J., 1983: Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. A direct enzyme-linked immunosorbent assay (ELISA) is described that uses horseradish peroxidase-labeled antigen for detection of immunoglobulin M (IgM) and IgA antibodies to toxoplasma. In this assay, polystyrene microtiter plates were sensitized...

Herbrink, P.; van Loon, A.M.; Rotmans, J.P.; van Knapen, F.; van Dijk, W.C., 1987: Interlaboratory evaluation of indirect enzyme-linked immunosorbent assay, antibody capture enzyme-linked immunosorbent assay, and immunoblotting for detection of immunoglobulin M antibodies to Toxoplasma gondii. Serum samples from healthy laboratory personnel and 50 consecutive samples from 19 patients with anamnestic clinical signs of toxoplasmosis were assayed by 4 laboratories in the Netherlands for IgM antibodies to Toxoplasma gondii by an indirect EL...

Pappas M.G.; Hajkowski R.; Cannon L.T.Sr; Hockmeyer W.T., 1984: Dot enzyme linked immunosorbent assay comparison with standard enzyme linked immunosorbent assay and complement fixation assays for the diagnosis of human visceral leishmaniasis. Veterinary Parasitology 14(3-4): 239-250

Yang, B.; Wang, C.; Chen, S.; Chen, X.; Lu, X.; Tian, H.; Yu, M.; Zhang, D.; Shi, Z.; Zhou, G.; Zhang, F., 2013: Evaluation of the combination of BP180-NC16a enzyme-linked immunosorbent assay and BP230 enzyme-linked immunosorbent assay in the diagnosis of bullous pemphigoid. Bullous pemphigoid (BP) is an acquired autoimmune subepidermal blistering disease characterized by circulating IgG autoantibodies directed against BP180 and BP230 hemidesmosomal proteins. Previous studies have demonstrated that antibodies against...

Sacks, J.M.; Gillette, K.G.; Frank, G.H., 1988: Development and evaluation of an enzyme-linked immunosorbent assay for bovine antibody to Pasteurella haemolytica: constructing an enzyme-linked immunosorbent assay titer. A 2-stage strategy was developed and evaluated for estimating serum antibody titre by use of ELISA and a series of dilutions. In stage 1, the linear response region and least-square estimate of the assay line slope were established from 9-point di...

Lope Pihie, A.H.; Phuah, K.O., 1996: Enzyme-linked immunosorbent assay for carbaryl in vegetables. An ELISA assay to detect carbaryl residues in vegetables is described.

Kumar, N.; Ghosh, S.; Gupta, S.C., 2008: Early detection of Fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay and dot enzyme-linked immunosorbent assay. In an attempt to develop a suitable serological test for early detection of <i>Fasciola gigantica</i> infection in buffaloes, a group of proteins were isolated from the somatic antigen of the parasite by immunoaffinity chromatography....