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Estradiol binding sites from mung bean hypocotyls

, : Estradiol binding sites from mung bean hypocotyls. Acta Phytophysiologica Sinica 18(1): 100-104

Different fractions of extracts from etiolated mung bean hypocotyls were tested for estradiol binding. Among them, the 1000 .apprx. 10,000 .times. g fraction was found to have stronger estradiol binding than other fractions. The estradiol binding to 1000 .apprx. 10,000 .times. g fractions was saturated after incubation for 1 h. The binding was temperature-dependent, showing a maximum at C. In a relatively wide pH range of 5 .apprx. 8.5, the estradiol binding was stable and the optimal pH was 7.5. Trypsin treatment lowered the estradiol binding indicating that estradiol binding sites were proteinous in nature. Some steroids other than estradiol could also bind to some extent to the estradiol binding sites. By Scatchard analysis, the number of estradiol binding sites was determined to be 29 .apprx. 108 fmol/mg protein and the dissociation constant (Kd) was 11 .apprx. 20 nmol/L (n = 3).

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