EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Estradiol binding sites from mung bean hypocotyls






Acta Phytophysiologica Sinica 18(1): 100-104

Estradiol binding sites from mung bean hypocotyls

Different fractions of extracts from etiolated mung bean hypocotyls were tested for estradiol binding. Among them, the 1000 .apprx. 10,000 .times. g fraction was found to have stronger estradiol binding than other fractions. The estradiol binding to 1000 .apprx. 10,000 .times. g fractions was saturated after incubation for 1 h. The binding was temperature-dependent, showing a maximum at 4.degree. C. In a relatively wide pH range of 5 .apprx. 8.5, the estradiol binding was stable and the optimal pH was 7.5. Trypsin treatment lowered the estradiol binding indicating that estradiol binding sites were proteinous in nature. Some steroids other than estradiol could also bind to some extent to the estradiol binding sites. By Scatchard analysis, the number of estradiol binding sites was determined to be 29 .apprx. 108 fmol/mg protein and the dissociation constant (Kd) was 11 .apprx. 20 nmol/L (n = 3).


Accession: 002373545



Related references

Zhang, J.S.ng; Yang, Z.H.n; Cao, Z.X.n, 1994: Partial purification and characterization of estrogen-binding proteins from mung bean hypocotyls. From the 10000 times g fraction of mung bean (Phaseolus aureus) hypocotyls, the estradiol-binding proteins were solubilized with NaClO-4 and partially purified by Sephadex G-100 column chromatography and native PAGE. The results showed that there...

Nakajima, M.; Sakai, S.; Kanazawa, K.; Kizawa, S.; Yamaguchi, I.; Takahashi, N.; Murofushi, N., 1993: Partial purification of a soluble gibberellin-binding protein from mung bean hypocotyls. A soluble binding protein specific for GA-4, GA-7 and GA-9 was partially purified from mung bean (Vigna ratiata) hypocotyls, and its characteristics were examined. Affinity chromatography using immobilized GA-3 coupled to Sepharose 4B via the C-7...

Basel, L.E.; Cleland, R.E.; Cleland, R.E., 1992: Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls. A comparison has been made of the developmental gradients along a mung bean (Vigna radiata L.) hypocotyl of the growth rate, plasma membrane ATPase, and fusicoccin-binding protein (FCBP) activity to determine whether they are interrelated. The hoo...

Basel, L.; Cleland, R., 1992: Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls. A comparison has been made of the developmental gradients along a mung bean (Vigna radiata L.) hypocotyl of the growth rate, plasma membrane ATPase, and fusicoccin-binding protein (FCBP) activity to determine whether they are interrelated. The hoo...

Kasamo, K.; Yamaki, T., 1976: In vitro binding of IAA to plasma membrane-rich fractions containing Mg++-activated ATPase from mung bean hypocotyls. Stepwise sucrose density-gradient centrifugation gave 6 fractions from homogenates of etiolated mung bean [Vigna mungo] hypocotyls. The distributions of ATPase, vesicular membranes and plasma membranes among fractions and the binding of 14C-IAA to...

Sugaya, S.; Sakai, S., 1996: A soluble auxin-binding protein from mung bean hypocotyls has indole-3-acetaldehyde reductase activity. To clarify the roles of auxin-binding proteins (ABPs) in the action of auxin, soluble auxin-binding proteins were isolated from an extract of etiolated mung bean hypocotyls by affinity chromatography on 2,4-dichlorophenoxyacetic acid (2,4-D)-linke...

Kasamo, K.; Yamaki, T., 1976: In vitro binding of iaa to plasma membrane rich fractions containing magnesium activated atpase from mung bean hypocotyls. Cell homogenates of dark-grown mung bean [Phaseolus mungo] hypocotyls were fractionated into 6 fractions (L-0, L-1 to L-5) by stepwise sucrose density-gradient centrifugation. The majority (about 84%) of Mg2+-activated ATPase activity of the 10,00...

Yamanishi, H.; Kasamo, K., 1992: Binding of 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole to an Essential Cysteine Residue(s) in the Tonoplast H-ATPase from Mung Bean (Vigna radiata L.) Hypocotyls. Vacuolar-type H(+)-ATPase was solubilized from tonoplasts of mung bean (Vigna radiata L.) and purified on a Mono Q anion-exchange column by fast protein liquid chromatography. The purified enzyme was inactivated by the reactive adenine analog, 7-c...

Yamanishi, H.; Kasamo, K., 1992: Binding of 7 chloro 4 nitrobenzo 2 oxa 1 3 diazole to an essential cysteine residues in the tonoplast proton atpase from mung bean vigna radiata l. hypocotyls. Vacuolar-type H+-ATPase was solubilized from tonoplasts of mung bean (Vigna radiata L.) and purified on a Mono Q anion-exchange column by fast protein liquid chromatography. The purified enzyme was inactivated by the reactive adenine analog, 7-chl...

Basel, L.E.; Zukowski, A.T.; Cleland, R.E., 1994: Modulation of Fusicoccin-Binding Protein Activity in Mung Bean (Vigna radiata L.) Hypocotyls by Tissue Maturation and by Fusicoccin. The phytotoxin fusicoccin (FC), after binding to a plasma membrane-localized receptor, causes higher plant cells to excrete protons. Ligand-binding analysis has been used to show that the plasma membrane of mung bean (Vigna radiata L.) hypocotyls...