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Estrogen mediated bovine mammary epithelial cell proliferation: evidence of indirect action


, : Estrogen mediated bovine mammary epithelial cell proliferation: evidence of indirect action. Journal of Dairy Science 75(Supplement 1): 293

To explain acute oestradiol-17 beta (E)-induced mammary cell proliferation in heifers (see Journal of Dairy Science (1991) 74 (Supplement 1) 295), bovine mammary epithelial cells (MAC-T) cells were placed onto 24-well culture dishes (20 000 per well/ml) in DMEM with 10% FCS for 24 h and test media (duplicate trials) applied until quantification of DNA after 72 h. E (0.1, 1, 10 and 100 ng/ml) alone, with 1% FCS, with progesterone (P) (0.5 and 5 ng/ml), or 1% FCS failed to stimulate growth.

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