EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Estrogen receptor binding in the hen hypothalamus and pituitary during the ovulatory cycle


Poultry Science 72(5): 839-847
Estrogen receptor binding in the hen hypothalamus and pituitary during the ovulatory cycle
The equilibrium dissociation constant (Kd) and the maximum binding capacity (Bmax) of estrogen receptor in soluble (cytosolic) and insoluble (nuclear) fractions in a hypotonic buffer solution of hypothalamus containing preoptic (HPOA) and median eminence (HMEA) areas and of anterior pituitary (AP) of laying and nonlaying hens were examined by Scatchard analysis of specific (3H)estradiol-17-beta((3H)-E-2)binding. The Kd of receptor in all of the tissues was different neither between soluble and insoluble fractions, nor between laying and nonlaying hens. The Bmax in laying hens was greater in the insoluble fraction and lower in the soluble fraction than that in nonlaying hens, but the total binding capacity (sum of Bmax in the soluble and insoluble fractions) was not different between laying and nonlaying hens. In laying hens, the specific (3H)-E-2 binding in the insoluble fraction of HPOA was found to increase at 21 h before ovulation and again at 8 to 6 h before ovulation, and of HMEA and AP at 18 to 11 h before ovulation. No change in the specific (3H)-E-2 binding in the insoluble and soluble fractions was found in any of the tissues of nonlaying hens during a 24-h period. The results suggest that in laying hens, estrogen may act on the hypothalamus and pituitary at restricted hours during the ovulatory cycle.


Accession: 002373559

PMID: 8502606

DOI: 10.3382/ps.0720839



Related references

Relationship between the changes in cytoplasmic progesterone receptor concentration and in nuclear progesterone binding sites in the hen hypothalamus and pituitary during the ovulatory cycle. Endocrinologia Japonica 27(5): 667-670, 1980

Changes in progesterone receptor binding of preoptic hypothalamus during an ovulatory cycle of the hen. Poultry Science 73(6): 855-863, 1994

Cytoplasmic progesterone receptor concentrations in the hen hypothalamus and pituitary: difference between laying and nonlaying hens and changes during the ovulatory cycle. Biology of Reproduction 20(3): 581-585, 1979

Expression of estrogen receptor alpha and beta genes in the mediobasal hypothalamus, pituitary and ovary during the canine estrous cycle. Neuroscience Letters 347(2): 131-135, 2003

Estrogen receptor dynamics in rat hypothalamus and pituitary gland after the injection of estrogen catechol estrogen or anti estrogen. Acta Endocrinologica Supplementum 99(246): 51-52, 1982

Effects of 17 beta-estradiol, synthetic estrogens, and antiestrogens on prolactin secretion, nuclear translocation of pituitary estrogen receptor and on progesterone receptor levels in rat pituitary, hypothalamus and uterus. Endocrinologia Experimentalis 15(1): 35-44, 1981

Correlation between LH and estrogen receptor turnover in pituitary and hypothalamus of castrate rats following estrogen agonists and antagonists. Journal of Steroid Biochemistry 24(2): 623-628, 1986

Correlation between LH and estrogen receptor turnover in pituitary and hypothalamus of castrate rats following estrogen agonists and antagonists. Journal of Steroid Biochemistry 24(2): 623-628, 1986

Estrogen and antiestrogen binding to rat uterine and pituitary estrogen receptor evidence for at least two physicochemical forms of the estrogen receptor. Journal of Steroid Biochemistry 23(5 PART A): 537-546, 1985

Neonatal imprinting and regulation of estrogen receptor alpha and beta mRNA expression by estrogen in the pituitary and hypothalamus of the male rat. Neuroendocrinology 73(1): 12-25, 2001