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Ethanol ingestion combined with lowered carbohydrate intake enhances the initiation of diethylnitrosamine liver carcinogenesis in rats

, : Ethanol ingestion combined with lowered carbohydrate intake enhances the initiation of diethylnitrosamine liver carcinogenesis in rats. Nutrition and Cancer 17(2): 171-178

The effect of ethanol on the initiation of diethylnitrosamine- (DEN) induced liver carcinogenesis was investigated in rats. In the first experiment, eight-week-old male Wistar rats were maintained on four liquid diets: a basal diet (Group 1), a low-carbohydrate (low-CHO) diet (Group 2), a basal diet + ethanol (Group 3), or a low-CHO diet + ethanol (Group 4). After three weeks on these diets, 50 mg/kg of DEN was injected intraperitoneally. The plasma glutamic-oxaloacetic transaminase activity in Group 4 was higher 24 hours after DEN administration than in Groups 1 and 3. The plasma glutamic-pyruvic transaminase activity in Groups 3 and 4 was higher than in Groups 1 and 2. The number of .gamma.-glutamyl transpeptidase-positive foci per unit liver area 41 weeks after DEN administration was higher in group 4 than in Group 1. The area of .gamma.-glutamyl transpeptidase-positive foci was greater in Groups 2 and 4 than in Group 1. In the second experiment, Groups 1 and 4 were given DEN orally (25 or 75 mg/kg). Plasma glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase activities 24 hours after DEN administration were significantly higher in Group 4 than in Group 1, but only when the dose of DEN was 75 mg/kg. In contrast, the number and area of placental glutathione-S-transferase-positive foci per unit liver area were greater in Group 4 than in Group 1 only after 25 mg/kg of DEN. Thus the severity of hepatotoxicity and the incidence of precancerous liver lesions were not necessarily correlated. These findings together indicate that a combination of ethanol and a low-CHO diet enhances DEN-induced liver carcinogenesis in rats by increasing the bioactivation of DEN in the liver.

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Accession: 002373610

PMID: 1350084

DOI: 10.1080/01635589209514184

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Gallinelli, R.; Coppini, D., 1965: A new iron chelating agent: desferrioxamine B (Desferal). Clinico-pharmacological observations. Cases. Minerva Medica 56(92): 3980-3985

Harvey G.J., 1986: A fast simple method for analysis of phenoxy acid salt and ester formulations by high performance liquid chromatography. Salt formulations of 2,4-D (2,4-dichlorophenoxyacetic acid), 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) and dichlorprop [2-(2,4-dichlorophenoxy)propanoic acid] have been analysed by reversed-phase HPLC using a C18-column with 50:50 (v/v) acetonit...

Longo, F.J., 1973: An ultrastructural analysis of polyspermy in the surf clam, Spisula solidissima. The response of a transplantable rat fibrosarcoma, RIB(5), to a single injection of 100 mg/kg body weight of cyclophosphamide, 0·5, 1, 2 and 3 days after treatment, has been studied by means of a morphometric analysis of tumour sections and has b...

Oberemm, A.; Kirschbaum, F., 1992: Valproic acid induced abnormal development of the central nervous system of three species of amphibians: Implications for neural tube defects and alternative experimental systems. Embryos of Ambrystoma mexicanum, Xenopus laevis, and Hyperolius viridiflavus taeniatus were exposed to various concentrations of valproic acid (VPA: 0.1, 1, 5, 10 mM) from blastula stage (S) 9 on up to advanced gastrulation of control embryos (S 1...

Osmond, D.L.; Xu, L.; Ranells, N.N.; Hodges, S.C.; Hansard, R.; Pratt, S.H., 2001: Nitrogen loss estimation worksheet (NLEW): an agricultural nitrogen loading reduction tracking tool. The Neuse River Basin in North Carolina (USA) was regulated in 1998, requiring that all pollution sources (point and nonpoint) reduce nitrogen (N) loading into the Neuse Estuary by 30%. Point source N reductions have already been reduced by approx...

Hatfield S.M.; Schmidtke J.R., 1980: Human thymus derived cell monocyte interactions in mitogenesis. Federation Proceedings 39(3): ABSTRACT 3405

Schneider, H., 1968: The intramembrane location of alcohol anesthetics. In order to identify the chemical nature of the membrane receptor sites involved in general anesthesia the apparent free energy of binding of methylene groups of n-alcohols in anesthetic systems was evaluated and compared with data from model syst...

Reid, R. A., 1975: Simulation and evaluation of an experimental rural medical care delivery system. Simulated results are given of four case studies reflecting the influence of alternative policies in a comparative framework of rural planning. The role of health in rural development is an important constituent policy that requires careful planni...

LEDVINKOVA.B.; HLINOVSKY.M., 1963: Preventive Examinations For Adolescents. Ceskoslovenske Zdravotnictvi 11: 179-181

Harrington, C.A.; Mobley, S.L.; Wenk, G.L., 1994: Nitric oxide formation does not underlie the memory deficits produced by ibotenate injections into the nucleus basalis of rats. Basal forebrain (BF) injections of ibotenic (IBO) acid impair memory, whereas quisqualic (QUIS) acid injections do not. The authors investigated whether the cytotoxicity and differential behavioral effects of IBO and QUIS in rats depend on the gen...