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Etiological studies of viral diseases of tomato in Malakand Agency of North West Frontier Province of Pakistan

, : Etiological studies of viral diseases of tomato in Malakand Agency of North West Frontier Province of Pakistan. Sarhad Journal of Agriculture 9(1): 27-35

Mosaic, mottle, rolling of leaves, leaf chlorosis, leaf malformations, fern leaf and shoe string, curling, bush growth and ring spot symptoms were predominantly recorded in winter tomato plants in Malakand Agency of North West Frontier Province (NWFP) Pakistan. Viruses tentatively diagnosed on the basis of these characteristics and typical symptoms were Tomato mosaic virus, cucumber mosaic virus, Tomato yellow leaf curl virus, Tomato yellow top virus, Potato leaf roll virus, Potato virus Y and X, Tomato bushy stunt virus, Tomato aspermy virus and Tomato ring spot virus. Both single virus and mixed virus infections were found. These viral infections were confirmed and verified through serological (Indirect enzyme-linked immunosorbent assay (ELISA) and double diffusion test) and biological (host range, transmission to indicator hosts and transmission properties) infectivity assay. Tomato mosaic virus was the most destructive and widely prevalent virus in the area.

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