+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of agents for use in medium for selective isolation of Lyme disease and relapsing fever Borrelia species



Evaluation of agents for use in medium for selective isolation of Lyme disease and relapsing fever Borrelia species



European Journal of Clinical Microbiology and Infectious Diseases 12(7): 512-518



Barbour-Stoenner-Kelly (BSK) II medium containing fosfomycin, 5-fluorouracil, trimethoprim and sulfamethoxazole was evaluated for the selective isolation of the Borrelia species responsible for Lyme disease and relapsing fever. The maximum non-inhibitory concentrations of fosfomycin, 5-fluorouracil, trimethoprim and sulfamethoxazole for six strains of borreliae were 500 to > 1000 micrograms/ml, 250 to > 500 micrograms/ml, 125 to 500 micrograms/ml and 125 to 500 micrograms/ml, respectively. The combination of four agents (fosfomycin 400 micrograms/ml, 5-fluorouracil 100 micrograms/ml, trimethoprim 10 micrograms/ml, sulfamethoxazole 50 micrograms/ml) did not inhibit the growth of borreliae, allowing growth in cultures inoculated with a few organisms (theoretically a single organism). In contrast, the four-agent combination completely inhibited the growth of 12 of 13 other bacterial strains tested as possible contaminants. This combination also allowed the selective growth of borreliae in experimentally contaminated specimens. The four-agent combination in BSK II medium may be useful for selective isolation of Borrelia species responsible for Lyme disease and relapsing fever from clinical and environmental samples.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002374565

Download citation: RISBibTeXText

PMID: 8404911


Related references

Borrelia sp. phylogenetically different from Lyme disease- and relapsing fever-related Borrelia spp. in Amblyomma varanense from Python reticulatus. Parasites and Vectors 9(1): 359, 2016

Characterization of reptile-associated Borrelia sp. in the vector tick, Amblyomma geoemydae, and its association with Lyme disease and relapsing fever Borrelia spp. Environmental Microbiology Reports 3(5): 632-637, 2011

A phylogenomic and molecular marker based proposal for the division of the genus Borrelia into two genera: the emended genus Borrelia containing only the members of the relapsing fever Borrelia, and the genus Borreliella gen. nov. containing the members of the Lyme disease Borrelia (Borrelia burgdorferi sensu lato complex). Antonie van Leeuwenhoek 105(6): 1049-1072, 2014

A lyme disease and relapsing fever mouse model use of the polymerase chain reaction for the detection of borrelia burgdorferi and borrelia hermsii in blood. Abstracts of the General Meeting of the American Society for Microbiology 91: 65, 1991

Borrelia infections Relapsing fever and lyme disease. Cunningham, M W , Fujinami, R S Effects of microbes on the immune system 57-70, 2000

P66 porins are present in both Lyme disease and relapsing fever spirochetes: a comparison of the biophysical properties of P66 porins from six Borrelia species. Biochimica et Biophysica Acta 1798(6): 1197-1203, 2010

Borrelia miyamotoi Disease: Neither Lyme Disease Nor Relapsing Fever. Clinics in Laboratory Medicine 35(4): 867-882, 2015

Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human. Bmc Research Notes 2: 134, 2009

Expression and localization analyses of the Borrelia Bdr proteins in Lyme disease and relapsing fever isolates. Abstracts of the General Meeting of the American Society for Microbiology 100: 274, 2000

Borrelia recurrentis characterization and comparison with relapsing-fever, Lyme-associated, and other Borrelia spp. International Journal of Systematic Bacteriology 47(4): 958-968, 1997

Hypercholesterolemia and ApoE deficiency result in severe infection with Lyme disease and relapsing-fever Borrelia. Proceedings of the National Academy of Sciences of the United States of America 112(17): 5491-5496, 2015

The loss and gain of marginal zone and peritoneal B cells is different in response to relapsing fever and Lyme disease Borrelia. Journal of Immunology 182(1): 498-506, 2009

Lyme disease and relapsing fever Borrelia elongate through zones of peptidoglycan synthesis that mark division sites of daughter cells. Proceedings of the National Academy of Sciences of the United States of America 113(33): 9162-9170, 2016

Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France. Parasites and Vectors 7: 233, 2014

The relapsing fever spirochete Borrelia hermsii contains multiple, antigen-encoding circular plasmids that are homologous to the cp32 plasmids of Lyme disease spirochetes. Infection and Immunity 68(7): 3900-3908, 2000