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Factors affecting protoplast isolation and the regeneration of shoot-like structures from protoplast-derived callus of sugarcane (Saccharum spp. hybrids)

Taylor, P.W.J.; Ko, H.L.; Adkins, S.W.

Australian Journal of Botany 40(6): 863-876

1992

ISSN/ISBN: 0067-1924
DOI: 10.1071/bt9920863
Accession: 002379893
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High yields of protoplasts were isolated from non-regenerable, homogeneous cell suspension cultures of sugarcane, compared with regenerable, heterogeneous cell suspension cultures after incubation in an enzyme composition containing Cellulase RS, Pectinase, Macerozyme and Driselase.

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