Factors affecting protoplast isolation and the regeneration of shoot-like structures from protoplast-derived callus of sugarcane (Saccharum spp. hybrids)
Taylor, P.W.J.; Ko, H.L.; Adkins, S.W.
Australian Journal of Botany 40(6): 863-876
High yields of protoplasts were isolated from non-regenerable, homogeneous cell suspension cultures of sugarcane, compared with regenerable, heterogeneous cell suspension cultures after incubation in an enzyme composition containing Cellulase RS, Pectinase, Macerozyme and Driselase.