Freezing preservation of dormant buds and callus of Populus koreana x P. nigra var. italica

Chung, H.G.; Kim, S.H.; Lee, S.K.

Research Report of the Institute of Forest Genetics 25: 142-148

1989


Accession: 002387209

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Abstract
Samples of Populus koreana x P. nigra var. italica bud and callus were treated with various cryopreservants (dimethylsulfoxide, glycerin, proline, alone or in combination, at various concentrations). After 60 d storage at temperatures of -45 degrees C, about 60% of samples survived and subsequently grew.