Frequency and spectrum of mutations induced by separate and simultaneous application of gamma rays and ethyl methane sulphonate (EMS) in two microsperma varieties of lentil

Tripathi, A.; Dubey, D.K.

Lens 19(1): 3-8

1992


Accession: 002387267

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Abstract
Seeds of two microsperma varieties of Lens culinaris were subjected to 5, 10 or 15 KR of gamma -rays, and/or 0.0125% of ethyl methanesulfonate (EMS). The varieties responded similarly to the mutagenic treatments regarding percentage of M2 families segregating for mutants, but differed regarding number of mutants/1000 M2 plants. Combined mutagenic treatments induced a higher percentage of families segregating for mutants than respective individual application of either mutagen in both varieties but the treatments did not show exact additive effects of the physical and chemical mutagens. In the variety K333, individual application of EMS induced a higher number of mutants/1000 M2 plants than either the gamma -ray treatments individually or the combined treatments. The same held true for variety K75, except that the number of mutants in combined treatments at the highest dose of irradiation exceeded that in the EMS treatment. Various chlorophyll, morphological and sterile mutants are described.