+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing

Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing

Plant Physiology 103(2): 359-370

Two hundred thirty-seven expressed sequence tags (ESTs) of Brassica napus were generated by single-run partial sequencing of 197 random root cDNA clones. A computer search of these root ESTs revealed that 21 ESTs show significant similarity to the protein-coding sequences in the existing data bases, including five stress- or defense-related genes and four clones related to the genes from other kingdoms. Northern blot analysis of the 10 data base-matched cDNA clones revealed that many of the clones are expressed most abundantly in root but less abundantly in other organs. However, two clones were highly root specific. The results show that generation of the root ESTs by partial sequencing of random cDNA clones along with the expression analysis is an efficient approach to isolate genes that are functional in plant root in a large scale. We also discuss the results of the examination of cDNA libraries and sequencing methods suitable for this approach.

(PDF emailed within 0-6 h: $19.90)

Accession: 002390002

Download citation: RISBibTeXText

PMID: 8029332

DOI: 10.2307/4275403

Related references

Development and application of single nucleotide polymorphism markers in the polyploid Brassica napus by 454 sequencing of expressed sequence tags. Plant Breeding 131(2): 293-299, 2012

Analysis of 176 expressed sequence tags generated from cDNA clones of hot pepper by single-pass sequencing. Journal Of Plant Biology. 41(2): 116-124, E, 1998

Characterization of expressed sequence tags generated from skin cDNA clones of Equus caballus by single pass sequencing. Animal Biotechnology 12(1): 87-97, 2001

Sequencing of cDNA clones randomly chosen from an immature flower cDNA library of Chinese cabbage: expressed sequence tags. RDA Journal of Agricultural Science, Biotechnology 37(1): 167-171, 1995

Generation of expressed sequence tags from 21 randomly selected cDNA clones derived from a bovine ovary cDNA library. Animal Genetics 27(SUPPL 2): 94, 1996

Sequencing of cDNA clones and analysis of the expressed sequence tags (ESTs) of tea plant young shoots. Chinese Journal of Agricultural Biotechnology 2(2): 137-141, 2005

Sequencing of cDNA clones and analysis of the expressed sequence tags (ESTs) properties of young tea plant (Camellia sinensis) shoots. Journal of Agricultural Biotechnology 13(1): 21-25, 2005

Generation and characterization of expressed sequence tags (ESTs) from coralloid root cDNA library of Cycas debaoensis. Plant Diversity 40(5): 245-252, 2019

Expressed sequence tags from the defense of Brassica napus to Leptosphaeria maculans. Molecular Plant Pathology On line: ( 0301 Fristensky), 1999

Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nature Genetics 4(4): 373-380, 1993

Mining expressed sequence tags of rapeseed (Brassica napus L.) to predict the drought responsive regulatory network. Physiology and Molecular Biology of Plants 21(3): 329-340, 2015

Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding. Genome 55(2): 118-133, 2012

Cloning and sequencing of a cDNA sequence encoding FAD2 from Brassica napus. Journal of Hunan Agricultural University 28(2): 97-99, 2002

A large scale analysis of cDNA in Arabidopsis thaliana: generation of 12,028 non-redundant expressed sequence tags from normalized and size-selected cDNA libraries. Dna Research 7(3): 175-180, 2000

Sequencing of cDNA clones and analysis of the expressed sequenced tags (ESTs) properties of male floral buds of Populus deltoides. Scientia Silvae Sinicae 42(11): 37-41, 2006