EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Generation of Large Numbers of Independently Transformed Fertile Barley Plants



Generation of Large Numbers of Independently Transformed Fertile Barley Plants



Plant Physiology 104(1): 37-48



A rapid, efficient, and reproducible system to generate large numbers of independently transformed, self-fertile, transgenic barley (Hordeum vulgare L.) plants is described. Immature zygotic embryos, young callus, and microspore-derived embryos were bombarded with a plasmid containing bar and uidA either alone or in combination with another plasmid containing a barley yellow dwarf virus coat protein (BYDVcp) gene. A total of 91 independent bialaphos-resistant callus lines expressed functional phosphinothricin acetyltransferase, the product of bar. Integration of bar was confirmed by DNA hybridization in the 67 lines analyzed. Cotransformation frequencies of 84 and 85% were determined for the two linked genes (bar and uidA) and for two unlinked genes (bar and the BYDVcp gene), respectively. More than 500 green, fertile, transgenic plants were regenerated from 36 transformed callus lines on bialaphos-containing medium; albino plants only were regenerated from 41 lines. T(0) plants in 25 lines (three plants per line) were analyzed by DNA hybridization, and all contained bar. Most contained the same integration patterns for the introduced genes (bar, uidA, and the BYDVcp gene) as their parental callus lines. Transmission of the genes to T(1) progeny was confirmed in the five families analyzed by DNA hybridization. A germination test of immature T(1) embryos on bialaphos-containing medium was useful for selecting individuals that were actively expressing bar, although this was not a good indicator of the presence or absence of bar. Expression of bar in some progeny plants was indicated by resistance to the herbicide Basta. The T(1) plants were in soil approximately 7 months after bombardment of the immature embryo.

(PDF same-day service: $19.90)

Accession: 002390007

Download citation: RISBibTeXText

PMID: 12232059

DOI: 10.2307/4275580



Related references

Generation of large numbers of independently transformed fertile perennial ryegrass (Lolium perenne L.) plants of forage- and turf-type cultivars. Molecular Breeding 6(5): 519-528, 2000

Transformed embryogenic microspores for the generation of fertile homozygous plants. Official Gazette of the United States Patent & Trademark Office Patents 1252(2), Nov 13, 2001

Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants. Plant Cell Reports 28(8): 1265-1272, 2009

Generation of large numbers of transgenic Kentucky bluegrass (Poa pratensis L.) plants following biolistic gene transfer. Plant Cell Reports 25(1): 19-25, 2005

Regeneration of fertile barley plants from protoplasts and production of transgenic barley by electroporation. VTT Publications 0(202): 1-95, 1994

Genetic modification of the waxy character in barley under the action of wild type exogenous dna analysis of the component composition of starch and the electrophoretic spectrum of grain hordein of transformed plants and the preservation of changes up to the 4th generation. Molekulyarnaya Biologiya (Moscow) 12(3): 637-645, 1978

Genetic modification of the waxy character in barley under the influence of wild type exogenous dna analysis of the composition of starch and the electrophoretic spectrum of hordein from caryopses of transformed plants and the retention of modifications up to the 4th generation. Molecular Biology (English Translation of Molekulyarnaya Biologiya (Moscow)) 12(3 PART 2): 485-490, 1978

Genetic modification of the waxy character in barley under the influence of wild type exogenous DNA. Analysis of the composition of starch and the electrophoretic spectrum of hordein from caryopses of transformed plants and the retention of modifications up to the fourth generation. Molecular biology 12(2): 485-490, 1978

Regeneration of fertile plants from transformed cells of immature arabidopsis thaliana l. heyhn. Genetika 28(3): 202-207, 1992

Generation of fertile transgenic barley synthesizing thermostable beta-amylase. Journal of the Institute of Brewing 103(3): 153, 1997

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof. Official Gazette of the United States Patent & Trademark Office Patents 1259(1), June 4, 2002

Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof. Official Gazette of the United States Patent & Trademark Office Patents 1219(4): 3314, Feb 23, 1999

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof. Official Gazette of the United States Patent & Trademark Office Patents 1231(3), Feb 15, 2000

Studies on fertile transgenic rice (Oryza sativa) plants transformed with AGP-ase gene and its economic characters. Acta Agriculturae Boreali Sinica 16(4): 11-14, 2001