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Generation of recombinant baculovirus via liposome-mediated transfection

Generation of recombinant baculovirus via liposome-mediated transfection

Biotechniques 11(3): 310, 312-3

Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, liposome-mediated transfection was found to be highly efficient.

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Accession: 002390014

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PMID: 1931027

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