EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Generation of viroid conformational isomers that are stable to incubation with magnesium ions and in a nuclear extract from tomato plants



Generation of viroid conformational isomers that are stable to incubation with magnesium ions and in a nuclear extract from tomato plants



Nucleic Acids Research 20(24): 6681-6686



We identified conditions for heating and quick cooling viroid RNAs in the presence of salt which lead to the production of conformational isomers stable to incubation for at least 45 minutes at 30 degrees in the presence of magnesium ions. Elution in 0.3 M NaCl allowed the purification of an electrophoretically slow form of an in vitro transcript carrying a complete copy of the potato spindle tuber viroid RNA sequence. Slow forms of this transcript and of kinase-labeled linear viroid RNA persisted for longer than 20 minutes when incubated with a protein-rich extract prepared from the nuclei of uninfected tomato plants, although both were slowly cleaved by a nuclease present in this extract. The fast form of the transcript was highly resistant to this tomato ribonuclease. The slow form of the transcript was much more susceptible to cleavage by RNase T1 than the fast form of this RNA, suggesting that the reduced gel mobility of the slow forms results from their relatively open structure. The ability to purify viroid conformational isomers from polyacrylamide gels will facilitate biochemical studies aimed at identifying host components interacting with RNAs of the viroid replication complex, which may not all be present in the most thermodynamically favored rodlike structure of mature viroids.

(PDF same-day service: $19.90)

Accession: 002390023

Download citation: RISBibTeXText

PMID: 1282703

DOI: 10.1093/nar/20.24.6681



Related references

Analysis of sequence polymorphism and population structure of tomato chlorotic dwarf viroid and potato spindle tuber viroid in viroid-infected tomato plants. Viruses 4(6): 940-953, 2012

Nuclear DNA from uninfected or potato spindle tuber viroid-infected tomato plants contains no detectable sequences complementary to cloned double-stranded viroid cDNA. Proceedings of the National Academy of Sciences of the United States of America 78(11): 6932-6935, 1981

Nuclear dna from uninfected or potato spindle tuber viroid infected tomato plants contains no detectable sequences complementary to cloned double stranded viroid complementary dna. Proceedings of the National Academy of Sciences of the United States of America 78(11): 6932-6935, 1981

Detection of Citrus exocortis viroid, Iresine viroid, and Tomato chlorotic dwarf viroid in new ornamental host plants in India. Plant Disease 90(11): 1457, 2006

Natural infections of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European journal of plant pathology 110(8): 823-831, 2004

Ecology of tomato plant macho viroid i. natural host plants agroecosystem effect on viroid incidence and influence of temperature on viroid distribution. Revista Mexicana de Fitopatologia 4(1): 19-28, 1986

Stimulation of a viroid like syndrome and the impairment of viroid infection in gynura aurantiaca plants by treatment with silver ions. Physiological & Molecular Plant Pathology 29(3): 317-324, 1986

Successive accumulation of two size classes of viroid-specific small RNA in potato spindle tuber viroid-infected tomato plants. Journal of General Virology 88(Pt 12): 3452-3457, 2007

High levels of viroid in tomato and potato plants inoculated with minimal amounts of potato spindle tuber viroid. Canadian Journal of Plant Pathology 12(1): 11-15, 1990

Linear oligomeric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells. Embo Journal 6(8): 2173-2183, 1987

Linear oligomeric potato spindle tuber viroid pstv rna species are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells. EMBO (European Molecular Biology Organization) Journal 6(8): 2173-2184, 1987

The influence of sodium, potassium and calcium ions on the influx and efflux of magnesium ions (magnesium 28) in intact plants and isolated roots. Landwirtschaftliche Forschung Sonderheft: ub 1975), 31 (1) 109-119, 1975

First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan. Journal Of General Plant Pathology: 2, 182-184, 2008

Transmission of Tomato chlorotic dwarf viroid by bumblebees in tomato plants. European Journal Of Plant Pathology6: 1, 111-115, 2010