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Genes to complex structures of bluetongue viruses: structure-function relationships of bluetongue virus proteins



Genes to complex structures of bluetongue viruses: structure-function relationships of bluetongue virus proteins



Bluetongue, African horse sickness, and related orbiviruses: Proceedings of the Second International Symposium: 383-389



cDNA clones of the dsRNA segments of bluetongue (BLU) viruses have been expressed in insect cell cultures using the baculovirus expression system. Co-expression of the inner capsid proteins VP3 and VP7 led to the formation of core-like particles (CLP), which were shown to consist of a subcore of VP3 surrounded by capsomeres of VP7. The 3 minor core proteins (VP1, VP4, and VP6) could also be incorporated into the CLP coexpression with VP3 and VP7.

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