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Genetic analysis and rapid mapping of a sporulation mutation in Magnaporthe grisea



Genetic analysis and rapid mapping of a sporulation mutation in Magnaporthe grisea



Molecular Plant-Microbe Interactions 7(1): 113-120



A developmental mutation causing abnormal conidium morphology and a reduction in sporulation was found in the rice blast fungus, Magnaporthe grisea. The conidium mutant, Con-, was recovered among survivors of an electroporation experiment in which germinated conidia of strain Guy11 were subjected to an electrical shock of 3,000 V/cm. Instead of producing a cluster of four or five conidia sympodially borne on a conidiophore, the Con- mutant produced an aerial hypha bearing an elongated terminal conidium (4 times 50 mu-m). The growth rate and sporulation of the Con- mutant on culture media were 73 and 2.3%, respectively, of that of the wild-type Guy11. Pigment formation in the mutant was reduced and delayed for at least 7 days relative to that of the wild-type strain. Random spore and tetrad analysis showed that the Con- phenotype was controlled by a single gene (Con1). Delayed pigment formation co-segregated with the Con- phenotype. The Con- strains did not produce normal appressoria on a siliconized slide or onion epidermis and were nonpathogenic on rice lines susceptible to the wild-type parent. Double mutants obtained from crosses between Con- strains and a previously described spore morphology (Smo) mutant had a Con- phenotype, indicating that the Con- mutation is epistatic to Smo-. As a first step toward physical isolation of the Con1 gene, bulked segregant analysis was employed to rapidly generate DNA markers flanking the Con1 gene. A linked marker 7 cM away from the Con1 locus was obtained.

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