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Genetic analysis of 11 polymorphic isozyme loci in chestnut species and characterization of chestnut cultivars by multi-locus allozyme genotypes



Genetic analysis of 11 polymorphic isozyme loci in chestnut species and characterization of chestnut cultivars by multi-locus allozyme genotypes



Journal of the American Society for Horticultural Science 119(4): 840-849



Allozyme polymorphism in chestnut (Castanea) species was investigated using isoelectric focusing in thin-layer polyacrylamide slab gets. Genetic analysis of the progenies of intraspecific crosses and interspecific F-2s and backcrosses (BC-1s) allowed the verification of 11 polymorphic isozyme loci from 11 enzyme systems. The following loci were defined: Acp, Adh, Est-1, Est-2, Est-5, Me, Prx-1, Prx-2, Prx-3, Skd-3, and Skd-4. All polymorphic loci behaved as single-locus Mendelian genes. Skd showed unique species specificity. Skd-1 and Skd-2 were unique to the American chestnut (C. dentata Borkh.) and the European chestnut (C. sativa Mill.), whereas Skd-3 and Skd-4 were unique to the Chinese chestnut (C. mollissima Bl.) and the Japanese chestnut (C. crenata Sieb.). Linkage anal vsis revealed linkage for three pairs of loci: Skd-3/Skd-4, Est-1/Est-2, and Est-5/Prx-1. The single-tree progeny method was used successfully for isozyme genetic analysis. Forty-seven chestnut cultivars in six chestnut species were characterized using 12 isozyme loci and can be unambiguously identified by 12 multi-locus genotypes. The interspecific and geographic relationships among species were also discussed.

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