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Genetic analysis of nucleotide triphosphatase activity in the mouse brain

Genetic analysis of nucleotide triphosphatase activity in the mouse brain

Genetics 137(1): 257-265

A Ca-2+- or Mg-2+-stimulated ecto-ATPase is thought to regulate the hydrolysis of extracellular ATP in nervous tissues. The hydrolysis of nucleotide triphosphates (NTPs) was analyzed in brain microsomal fractions from crosses of DBA/2J (D2) and C57BL/6J (B6) mice. The nucleotide triphosphatase (NTPase) activity was significantly reduced in D2 mice as compared to B6 mice, and B6D2F-1 hybrids had activities intermediate to the parentals. A significant positive correlation was found between the hydrolysis of four NTPs (ATP, CTP, GTP and UTP) in 24 B6 times D2 (BXD) recombinant inbred (RI) strains of mice and in 80 B6D2F-1 times D2 backcross mice. The RI strains and backcross mice fell into two distinctgroups with respect to the NTPase activity. Linkage of NTPase activity was suggested with the chromosome 2 markers, D2Mit6 and Ass-1, in the RI strains, and was confirmed by analysis of other markers in the backcross population. These data suggest that the Ca-2+- or Mg-2+-stimulated hydrolysis of NTPs, designated Ntp, is regulated by a single gene located on proximal chromosome 2. Although an association was observed previously between Ca-2+-ATPase activity and susceptibility to audiogenic seizures (AGS), no significant association was observed for the expression of Ntp and AGS susceptibility.

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Accession: 002390215

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PMID: 8056315

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