EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic analysis of nucleotide triphosphatase activity in the mouse brain



Genetic analysis of nucleotide triphosphatase activity in the mouse brain



Genetics 137(1): 257-265



A Ca-2+- or Mg-2+-stimulated ecto-ATPase is thought to regulate the hydrolysis of extracellular ATP in nervous tissues. The hydrolysis of nucleotide triphosphates (NTPs) was analyzed in brain microsomal fractions from crosses of DBA/2J (D2) and C57BL/6J (B6) mice. The nucleotide triphosphatase (NTPase) activity was significantly reduced in D2 mice as compared to B6 mice, and B6D2F-1 hybrids had activities intermediate to the parentals. A significant positive correlation was found between the hydrolysis of four NTPs (ATP, CTP, GTP and UTP) in 24 B6 times D2 (BXD) recombinant inbred (RI) strains of mice and in 80 B6D2F-1 times D2 backcross mice. The RI strains and backcross mice fell into two distinctgroups with respect to the NTPase activity. Linkage of NTPase activity was suggested with the chromosome 2 markers, D2Mit6 and Ass-1, in the RI strains, and was confirmed by analysis of other markers in the backcross population. These data suggest that the Ca-2+- or Mg-2+-stimulated hydrolysis of NTPs, designated Ntp, is regulated by a single gene located on proximal chromosome 2. Although an association was observed previously between Ca-2+-ATPase activity and susceptibility to audiogenic seizures (AGS), no significant association was observed for the expression of Ntp and AGS susceptibility.

(PDF emailed within 1 workday: $29.90)

Accession: 002390215

Download citation: RISBibTeXText

PMID: 8056315



Related references

In vivo effect of LSD, morphine, ethanol and delta9-tetrahydrocannabinol on mouse brain adenosine triphosphatase activity. Research Communications in Chemical Pathology and Pharmacology 7(1): 229-232, 1974

Effects of 5-phenyloxazolidinedione on sodium-potassium-magnesium-activated adenosine triphosphatase activity in mouse brain. Journal of Neurochemistry 19(7): 1783-1788, 1972

Nucleotide triphosphatase activity of the N-terminal nucleotide-binding domains of the multidrug resistance proteins P-glycoprotein and MRP1. Biochemical & Biophysical Research Communications 296(2): 388-394, August 16, 2002

Behavioral genetic analysis of regional mouse brain biogenic amines acidic metabolites and motor activity. Comparative Biochemistry & Physiology C Pharmacology Toxicology & Endocrinology 96(2): 389-392, 1990

Molecular analysis of mouse alcohol dehydrogenase: nucleotide sequence of the Adh-1 gene and genetic mapping of a related nucleotide sequence to chromosome 3. Gene 59(2-3): 171-182, 1987

Genetic analysis of a temperature-sensitive Salmonella typhimurium rho mutant with an altered rho-associated polycytidylate-dependent adenosine triphosphatase activity. Journal of Bacteriology 147(1): 13-24, 1981

Control of endogenous adenosine triphosphatase activity of pigeon heart mitochondria by energy-linked reduction of diphos-phopyridine nucleotide. II. The stoichiometry between adenosine triphosphate and reduced diphosphopyridine nucleotide. Journal of Biological Chemistry 238: 1516-1519, 1963

Genetic analysis of cyclic nucleotide phosphodiesterases in S49 mouse lymphoma cells. Advances in Cyclic Nucleotide and Protein Phosphorylation Research 16: 185-194, 1984

Nucleotide triphosphatase activity of red cells lysed by freezing. Int Congr Biochem 6(8): 649, 1964

The effect of temperature and 2,4-dinitrophenol on the nucleotide triphosphatase activity of myosin. BULL RES COUNC ISRAEL SECT A CHEM 9A(l/2): 100, 1960

Immunohistochemical analysis of the localization of guanine nucleotide-binding protein in the mouse brain. Brain Research 442(2): 305-311, 1988