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Genetic analysis of shoot regeneration in stem and hypocotyl cultures of Petunia



Genetic analysis of shoot regeneration in stem and hypocotyl cultures of Petunia



Japanese Journal of Breeding 43(2): 201-206



Differential responses of shoot regeneration to the media containing two different concentration of EDTA and the genetic factors on the shoot regeneration were studied in 3 lines of Petunia hybrida. On the medium containing 0.1 mM EDTA, vigorous shoot formation was observed in 'Violet' (V) and 'Mitchell Dihaploid' (MD). 'White' (W) produced few shoots. F-1 hybrids of V and W as well as V exhibited vigorous shoot formation. F-2 progenies of V times W and W times V gave a 3:1 segregation ratio of regenerated to non-regenerated explants indicating that V possessed a single dominant gene which induced vigorous shoot regeneration on the standard MS medium. On the medium with 0.3 mM EDTA, vigorous shoot formation was again exhibited by MD, while few shoots were noted in V. The reciprocal F-1 hybrids between V and MD regenerated shoots vigorously as in MD. In their F-2 progenies, the segregation ratio of many shoots (regenerated more than 20 shoots) and few shoots (less than 20 shoots) formed in hypocotyl explants fitted the 3:1 ratio. This indicates that MD might have a single dominant gene for tolerance to high EDTA concentration. These results suggest that shoot regeneration in MD, V, and W is controlled by different genes.

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Accession: 002390244

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