+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic analysis of stunted growth by nuclear-cytoplasmic interaction in interspecific hybrids of Capsicum by using RAPD markers

Genetic analysis of stunted growth by nuclear-cytoplasmic interaction in interspecific hybrids of Capsicum by using RAPD markers

Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 87(4): 416-422

When eight cultivars of Capsicum annuum were used as female parents in interspecific crosses with two accessions of C. chinense, dwarfism occurred in hybrids originating from 10 out of 16 combinations, while hybrids of the remaining 6 combinations grew normally. In contrast, when C. chinense was used as female parent, all of the hybrids showed severely stunted growth as if affected by a virus. These results suggested that the stunted growth expressed in the cross of C. chinense X C. annuum is caused by an interaction between nuclear gene(s) from C. annuum and the cytoplasm of C. chinense. To examine the number of nuclear gene(s) which cause(s) the stunted growth, we backcrossed F1 hybrids of C. annuum X C. chinense to C. chinense. About one-quarter of the progeny in the backcrossed hybrids of C. chinense X (C. annuum X C. chinense) showed the same stunted growth shown by the F1 hybrids of C. chinense X C. annuum, suggesting that two complementary genes of C. annuum cause the stunted growth. However, the higher abortion rates of ovules and lower germination percentage of seeds in C. chinense X C. annuum than in the selfed C. chinense implied that the genetic ratio of the stunted type would have been higher than that observed in the C. chinense X (C. annuum X C. chinense) progeny. We then attempted a linkage analysis between the stunted growth and randomly amplified polymorphic DNA (RAPD) of C. chinense X (C. annuum X C. chinense) progeny. A RAPD marker that associated with 94% of the stunted plants but not with 94% of the normal one was identified. This confirmed that a single nuclear gene of C. annuum which is linked to the RAPD marker with a recombination value of 6% causes the stunted growth in an interaction with the cytoplasm of C. chinense.

(PDF emailed within 0-6 h: $19.90)

Accession: 002390250

Download citation: RISBibTeXText

PMID: 24190313

DOI: 10.1007/BF00215086

Related references

Analysis of genetic variation in interspecific hybrids between Gossypium hirsutum and G. raimondii and their derivatives using RAPD markers. Journal of Huazhong Agricultural University 19(6): 523-527, 2000

RAPD markers for evaluating genetic diversity and identifying interspecific hybrids in citrus. Revista Brasileira de Fruticultura 22(1): 14-19, 2000

Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 92(8): 1091-1098, 1996

Progeny analysis of the interspecific somatic hybrids nicotiana tabacum cytoplasmic male sterile plus nicotiana sylvestris with respect to nuclear and chloroplast markers. Theoretical & Applied Genetics 56(4): 145-150, 1980

Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis. Acta Biochimica Polonica 61(2): 195-199, 2015

Assessment of random amplified polymorphic DNA (RAPD) as genetic markers for determining the origin of interspecific lilac hybrids. Taxon 42(3): 531-537, 1993

Molecular identification and the features of genetic diversity in interspecific hybrids of Amur sturgeon based on variability of multilocus RAPD markers. Russian Journal Of Genetics: 11, 1258-1265, 2008

Genetic analysis of Jatropha species and interspecific hybrids of Jatropha curcas using nuclear and organelle specific markers. Euphytica 168(2): 197-214, 2009

Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers. Plant Cell Reports 35(6): 1345-1358, 2016

A study of selected isozymes in Capsicum baccatum, Capsicum eximium, Capsicum cardenasii and two interspecific F1 hybrids in Capsicum species. Turkish Journal of Botany 24(6): 311-318, 2000

Genetic analysis of Chrysanthemum hybrids based on RAPD molecular markers. Botanical Bulletin of Academia Sinica 41(4): 257-262, 2000

Inheritance of RAPD markers in the F-1 interspecific hybrids of rice. Pakistan Journal of Botany 28(1): 51-59, 1996

Using RAPD molecular markers for the analysis of genetic variation of Gerbera cultivars and hybrids. Journal of the Agricultural Association of China 6(2): 161-176, 2005

Identification of hybrids from interspecific crosses in citrus using RAPD and SSR markers. Laranja 22(1): 231-241, 2001

Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers. Genetics and Molecular Research 12(1): 256-269, 2013