+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Genetic and phenotypic studies of hypomorphic lin-12 mutants in Caenorhabditis elegans

Genetic and phenotypic studies of hypomorphic lin-12 mutants in Caenorhabditis elegans

Genetics 135(3): 755-763

The lin-12 gene of Caenorhabditis elegans is thought to encode a receptor for intercellular signals that specify certain cell fates during development. We describe several alleles of lin-12 that reduce but do not eliminate lin-12 activity (hypomorphic alleles). These alleles cause a novel egg-laying defective (Egl) phenotype in hermaphrodites as well as incompletely penetrant cell fate transformations seen with high penetrance in lin-12 null mutants. Characterization of the Egl phenotype revealed additional roles of lin-12 in the development of the egg-laving system that were not apparent from studying lin-12 null mutants: lin-12 activity is required for proper early vulval morphogenesis as well as for some unknown later aspect of egg-laying system development. Reversion of the Egl phenotype caused by one lin-12 hypomorphic allele was used to identify potential interacting genes as described in the accompanying paper.

(PDF emailed within 1 workday: $29.90)

Accession: 002390389

Download citation: RISBibTeXText

PMID: 8293977

Related references

Genetic and Phenotypic Characterization of Roller Mutants of CAENORHABDITIS ELEGANS. Genetics 95(2): 317-339, 1980

Embryonic (and other) developmental temperature sensitive mutants of Caenorhabditis elegans - phenotypic and genetic characterization. Journal of Supramolecular Structure (52): 326, 1978

Lifespan extension in hypomorphic daf-2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2-2. Aging Cell 4(6): 299-307, 2005

Functional and phenotypic relevance of differentially expressed proteins in calcineurin mutants of Caenorhabditis elegans. Proteomics 6(4): 1340-1350, 2006

Functional phenotypic rescue of Caenorhabditis elegans neuroligin-deficient mutants by the human and rat NLGN1 genes. Plos One 7(6): E39277-E39277, 2012

Phenotypic and suppressor analysis of defecation in clk-1 mutants reveals that reaction to changes in temperature is an active process in Caenorhabditis elegans. Genetics 159(3): 997-1006, 2001

A genetic screen for cytokinesis mutants of Caenorhabditis elegans. Molecular Biology of the Cell 6(SUPPL ): 42A, 1995

Genetic and molecular analysis of heterochronic mutants in caenorhabditis elegans. Journal of Cellular Biochemistry Supplement (13 PART C): 255, 1989

A genetic selection for Caenorhabditis elegans synaptic transmission mutants. Proceedings of the National Academy of Sciences of the United States of America 93(22): 12593-8, 1996

Genetic and phenotypic analysis of spd-3 A mitotic spindle defective mutant in Caenorhabditis elegans. Molecular Biology of the Cell 11(Supplement): 170a, 2000

Genetic mapping of Caenorhabditis elegans collagen genes using DNA polymorphisms as phenotypic markers. Genetics 109(3): 513-528, 1985

Molecular, genetic and physiological characterisation of dystrobrevin-like (dyb-1) mutants of Caenorhabditis elegans. Journal of Molecular Biology 307(1): 107-117, 2001

Genetic analysis of temperature-sensitive embryogenesis mutants in Caenorhabditis elegans. Developmental Biology, 841: 193-205, 1981

A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans. G3 6(8): 2407-2419, 2016

Genetic analysis of sterile mutants in the dpy-5 unc-13 genomic region of Caenorhabditis elegans. Molecular & General Genetics 255(1): 60-77, 1997