EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas



Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas



Proceedings of the National Academy of Sciences of the United States of America 89(1): 43-46



The identification of apparently fastidious microorganisms is often problematic. DNA from a rickettsia-like agent (called the ELB agent) present in cat fleas could be amplified by PCR with conserved primers derived from rickettsial 17-kDa common protein antigen and citrate synthase genes but not spotted fever group 190-kDa antigen gene. Alu I sites in both the 17-kDa and citrate synthase PCR products obtained with the rickettsia-like agent and Rickettsia typhi were different even though both agents reacted with monoclonal antibodies previously thought specific for R. typhi. The DNA sequence of a portion of the 17-kDa PCR product of the rickettsia-like agent differed significantly from all known rickettsial sequences and resembled the 17-kDa sequences of typhus more than spotted fever group rickettsiae. The rare stable transovarial maintenance of this rickettsia in cat fleas has important implications for the disease potential of cat fleas.

(PDF same-day service: $19.90)

Accession: 002390483

Download citation: RISBibTeXText

PMID: 1729713

DOI: 10.2307/2358471



Related references

Transovarial transmission of murine typhus rickettsiae in Xenopsylla cheopis fleas. Science 227(4686): 543-545, 1985

Suppression of Rickettsia typhi transmission in fleas maintained on murine typhus-immune rats. American Journal of Tropical Medicine and Hygiene 37(3): 629-635, 1987

Ultrastructural and genetic evidence of a reptilian tick, Aponomma hydrosauri, as a host of Rickettsia honei in Australia: possible transovarial transmission. Annals of the New York Academy of Sciences 990: 67-74, 2003

Studies on the murine origin of typhus epidemics in North China II Typhus rickettsia isolated from mice and mouse-fleas during an epidemic in a household and from body lice in the garments of one of the epidemic cases. 1941

Transovarial transmission of Rickettsia-like microorganisms in mosquitoes. Annals of the New York Academy of Sciences 266: 152-161, 1975

Lack of transovarial transmission of Bartonella by rodent fleas. Molecular Ecology 20(13): 2660-2661, 2012

Lack of transovarial transmission of Bartonella by rodent fleas. Molecular Ecology 20.13: 2660-2661, 2011

Occurrence of a typhus like rickettsia associated with opossums and their fleas in los angeles county. American Journal of Tropical Medicine & Hygiene 45(3 SUPPL): 111-112, 1991

Typhus Rickettsia isolated from mice and mouse-fleas during an epidemic in Peiping. Proc Soc Exp Biol And Med 45(3): 823-826, 1940

Hemocytic rickettsia-like organisms in Ixodes scapularis: transovarial and transstadial transmission. Canadian Journal of Zoology. July; 737: 1380-1383, 1995

Isolation and characterization of a novel Rickettsia species (Rickettsia asembonensis sp. nov.) obtained from cat fleas (Ctenocephalides felis). International Journal of Systematic and Evolutionary Microbiology 66(11): 4512-4517, 2016

Efficiency of transovarial transmission of Rickettsia tsutsugamushi in Leptotrombidium arenicola (Acari: Trombiculidae). Journal of Medical Entomology 13(4-5): 493-496, 1977

Transovarial transmission of Rickettsia spp. and organ-specific infection of the whitefly Bemisia tabaci. Applied and Environmental Microbiology 78(16): 5565-5574, 2012