EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification



Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification



Phytopathology 83(9): 1003-1007



Strains of Fusarium graminearum were characterized using random amplified polymorphic DNA (RAPD) and restriction analysis of amplified fragments from the polymerase chain reaction (PCR). For RAPD analysis, three of 40 oligonucleotide primers were selected after testing with two F. graminearum strains and used to characterize an additional 17 strains of F. graminearum. For PCR amplifications, two sets of primers were designed from the sequence of cloned DNA fragments specific to F. graminearum. Most RAPD primers or PCR primer pairs yielded one of two common patterns, but the strains could be identified by the combined profile of patterns. The RAPD and PCR primers generally did not react with other Fusarium species or gave distinctly different patterns. The results suggest a relatively low amount of genetic diversity among the F. graminearum strains tested. The RAPD and specific PCR profiles are now being used for tracking strains of F. graminearum in field experiments.

(PDF 0-2 workdays service: $29.90)

Accession: 002390485

Download citation: RISBibTeXText



Related references

RAPD-PCR analysis of genetic variation among isolates of Fusarium graminearum and Fusarium culmorum from wheat in Adana Turkey. Pakistan Journal of Biological Sciences 13(3): 138-142, 2010

Genetic characterization of Fusarium oxysporum f. sp. vasinfectum isolates by random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 111(6): 534-544, 2004

Genetic diversity of Fusarium graminearum strains in eastern Canada. Phytoprotection 77(3): 140, 1996

Genetic diversity among Fusarium graminearum strains from Ontario and Quebec. Canadian journal of plant pathology = Revue Canadienne de phytopathologie 21(3): 308-314, 1999

Characterization and fitness of carbendazim-resistant strains of Fusarium graminearum (wheat scab). Pest Management Science 63(12): 1201-1207, 2007

Molecular characterization, fitness and mycotoxin production of Fusarium graminearum laboratory strains resistant to benzimidazoles. Pesticide Biochemistry and Physiology 128: 1-9, 2016

Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD). Current Genetics 27(6): 528-535, 1995

Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Parana, Brazil FT Compatibilidade vegetativa e caracterizacao molecular de isolados patogenicos de Fusarium graminearum do Estado, do Parana. 2007

Pathogenic and molecular characterization of Thielaviopsis paradoxa strains by RAPD and PCR amplification. Phytopathology 87(6 SUPPL ): S3, 1997

Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA. Genetics and Molecular Research 12(2): 1360-1372, 2013

Structural and functional characterization of the TRI101 trichothecene 3-O-acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: kinetic insights to combating Fusarium head blight. Journal of Biological Chemistry 283(3): 1660-1669, 2007

Researches on the microorganisms which deteriorate the stored cereals and grains xxxiv detection of injurious strains and properties of their toxic substance of scab fusarium blight grown on the wheat m fusarium graminearum fusarium nivale rice. Report of National Food Research Institute (Tokyo) (23): 89-116, 1968

Application of the random amplification of polymorphic DNA (RAPD) method in genetic monitoring of inbred mouse strains. Animal Science Papers and Reports 18(4): 269-275, 2000

Influence of temperature on zearalenone production by regional strains of Fusarium graminearum and Fusarium oxysporum in culture. International Journal of Food Microbiology 13(4): 329-333, 1991