EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic definition of a new bovine papillomavirus type 1 open reading frame, E5B, that encodes a hydrophobic protein involved in altering host-cell protein processing



Genetic definition of a new bovine papillomavirus type 1 open reading frame, E5B, that encodes a hydrophobic protein involved in altering host-cell protein processing



Journal of Virology 67(6): 3427-3434



We have previously observed that bovine papillomavirus type 1 (BPV-1) induces the appearance of five cellular proteins in C127 mouse fibroblasts, four of which appear to arise by altered processing or resident endoplasmic reticulum proteins. Studies of various cell lines revealed that expression of the 3' end of the BPV early region was sufficient for induction of these changes. To identify the BPV gene responsible, we have utilized the simian virus 40 (SV40)/BPV-1 recombinant virus Pava-1, which expresses the 3' end of the BPV early region behind an SV40 early promoter. C127 cells infected with Pava-1 for 48 h show the expected BPV-associated alterations, as do cells infected with Pava constructs mutated in the E5 or E2 genes. However, a mutation in the start codon of a previously ignored open reading frame extending from nucleotides 4013 to 4170 (E5B) eliminated the BPV-associated changes. Similar results were obtained with COS cells infected with the Pava mutants and C127 cells transformed by full-length mutated BPV. Despite its influence on the processing of cellular endoplasmic reticulum proteins, this mutation in E5B did not alter BPV-transforming efficiency or the ability of transformants to form colonies in soft agar. The E5B open reading frame encodes a hydrophobic 52-amino-acid polypeptide that shares structural similarities with HPV6 E5A and HPV16 E5. Speculations on a role for E5B in the viral life cycle are discussed.

(PDF 0-2 workdays service: $29.90)

Accession: 002390608

Download citation: RISBibTeXText

PMID: 8388507



Related references

The L2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens. Journal of Virology 60(2): 813-816, 1986

E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene. Journal of Virology 57(1): 1-6, 1986

The bovine herpesvirus type 1 UL3.5 open reading frame encodes a virion structural protein. Virology 240(1): 76-82, 1998

The e 5 open reading frame of bovine papillomavirus encodes a transforming gene. Journal of Cellular Biochemistry Supplement (10 PART A): 218, 1986

The E7 open reading frame of human papillomavirus type 16 encodes a transforming gene. Oncogene Research 3(2): 167-175, 1988

Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. Journal of General Virology 81(Pt 9): 2281-2287, 2000

Human papillomavirus type 16 open reading frame E7 encodes a transforming gene for rat 3Y1 cells. Journal of Virology 62(2): 610-613, 1988

Identification of B epitopes in human papillomavirus type 16 E7 open reading frame protein. Journal of General Virology 71: 1347-1354, 1990

Protein expression of human papillomavirus type 16 E-4 open reading frame from Escherichia coli. Protein Engineering 6(SUPPL ): 63, 1993

The herpes simplex virus type 1 UL3 transcript starts within the UL3 open reading frame and encodes a 224-amino-acid protein. Journal of Virology 81(19): 10524-10531, 2007

Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. Journal of Virology 68(6): 3917-3924, 1994

Equine herpesvirus type 1 (EHV-1) open reading frame 59 encodes an early protein that is localized to the cytosol and required for efficient virus growth. Virology 449: 263-269, 2014

Human adenovirus type 9 E4 open reading frame 1 encodes a cytoplasmic transforming protein capable of increasing the oncogenicity of CREF cells. Journal of Virology 70(2): 862-872, 1996

Mutational analysis of open reading frame E4 of bovine papillomavirus type 1. Journal of Virology 61(4): 1248-1252, 1987

A mutation in the latency-related gene of bovine herpesvirus 1 inhibits protein expression from open reading frame 2 and an adjacent reading frame during productive infection. Journal of Virology 78(6): 3184-3189, 2004