+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Genetic diversity of tall fescue germplasm based on RFLPs

Genetic diversity of tall fescue germplasm based on RFLPs

Crop Science 34(1): 246-252

The amount of genetic variation among and within tall fescue (Festuca arundinacea Schreb.) cultivars is an interest of geneticists and breeders. However, limited information is available on this. The objective of this study was to investigate the genetic diversity of tall fescue germplasm based on RFLP data. Twenty-four probes from a tall fescue PstI-genomic library were hybridized to EcoRI- or HindIII-digested genomic DNA from nine turf and seven forage cultivars, Twenty plants per cultivar were individually analyzed. Results showed variation within cultivars was high, varied among cultivars and probes, and increased with the number of parental clones (PN) up to 10 and then plateaued, indicating additional genetic variation could not be detected in cultivars with more than 10 parents. The number of bands was significantly different among cultivars and related to the within-cultivar genetic diversity, but not to the PN. Probes not hybridizing to the P genome detected less variation. Genetic distances calculated from 4, 8, 12, 16, and 20 plants were similar within cultivars, implying full random mating in tall fescue cultivar development. The between-cultivar genetic distances, however, decreased in larger sample sizes and became consistent when sample size reached 16 plants. Results suggested 16 randomly chosen plants was the minimum number to maintain adequate genetic diversity in these tall fescue cultivars. The associations of the 16 cultivars from cluster analysis agreed with available pedigree information. Turf cultivars tended to be clustered while forage cultivars were more divergent.

(PDF emailed within 1 workday: $29.90)

Accession: 002390785

Download citation: RISBibTeXText

Related references

Molecular characterisation and interpretation of genetic diversity within globally distributed germplasm collections of tall fescue (Festuca arundinacea Schreb.) and meadow fescue (F. pratensis Huds.). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 124(6): 1127-1137, 2012

Genetic diversity in a world germplasm collection of tall fescue. Genetics and Molecular Biology 36(2): 237-242, 2013

Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis. Plant Systematics and Evolution 282(1-2): 57-70, 2009

Genetic diversity among wild accessions and cultivars of Tunisian tall fescue based on morpho-agronomical traits and ISSR markers. Journal of Food Agriculture and Environment 10(1): 263-268, 2012

Genetic variation, heritability and genetic correlations for forage quality and yield traits of Mediterranean tall fescue germplasm. Plant breeding = Zeitschrift feur Pflanzenzuchtung 124(1): 99-101, 2005

Genetic diversity of oil palm germplasm collections using RFLPs. Cutting edge technologies for sustained competitiveness: Proceedings of the 2001 PIPOC International Palm Oil Congress, Agriculture Conference, Kuala Lumpur, Malaysia, 20-22 August 2001: 526-535, 2001

Effect of ploidy on quality of tall fescue italian ryegrass x tall fescue and tall fescue x giant fescue hybrids. Annals of Botany (London) 60(2): 127-132, 1987

Effect of Ploidy on Quality of Tall Fescue, Italian Ryegrass Tall Fescue and Tall Fescue Giant Fescue Hybrids. Annals of Botany 60(2): 127-132, 1987

Effects of genetic diversity on heterosis in tall fescue. Crop Science 13(1): 1-4, 1973

Effect of ploidy on quality of tall fescue, Italian ryegrass (times) tall fescue and tall fescue (times) giant fescue hybrids. Annals of Botany 60: 7-32, 1987

Relationships among European barley germplasm: I. Genetic diversity among winter and spring cultivars revealed by RFLPs. Crop Science 34(5): 1191-1199, 1994

Phylogeny of tall fescue and related species using RFLPs. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 88(6-7): 685-690, 1994

Effects of genetic diversity on response to heterosis in tall fescue. Agronomy Abstracts. 6-7, 1971

Determination of genetic diversity in tall fescue with AFLP markers. Crop Science 42(3): 944-950, 2002

Detection of RFLPs in perennial ryegrass, using heterologous probes from tall fescue. Crop Science 32(6): 1366-1370, 1992