Genetic fingerprinting of tobacco by RAPD. Use of markers to identify somatic hybrids between Nicotiana tabacum and Nicotiana rustica L

Coussirat, J.C.

Annales du Tabac Section 2 25: 1-6

1993


Accession: 002390909

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Abstract
Identification of the interspecific somatic hybrids was carried out utilizing the polymerase chain reaction and 4 primers of short sequence to direct amplification of DNA. Each primer gave a specific amplification of the genome and a characteristic fingerprint of both parents. The size of fragments of RAPD [random amplified polymorphic DNA] ranged from 100 to 2200 bp. The fingerprint of the somatic hybrids was similar to that of one of the parents or a combination of both. RAPD markers appeared a simple and rapid method for early selection of somatic hybrids.