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Genetic fingerprinting of tobacco by RAPD. Use of markers to identify somatic hybrids between Nicotiana tabacum and Nicotiana rustica L



Genetic fingerprinting of tobacco by RAPD. Use of markers to identify somatic hybrids between Nicotiana tabacum and Nicotiana rustica L



Annales du Tabac Section 2 25: 1-6



Identification of the interspecific somatic hybrids was carried out utilizing the polymerase chain reaction and 4 primers of short sequence to direct amplification of DNA. Each primer gave a specific amplification of the genome and a characteristic fingerprint of both parents. The size of fragments of RAPD (random amplified polymorphic DNA) ranged from 100 to 2200 bp. The fingerprint of the somatic hybrids was similar to that of one of the parents or a combination of both.

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Accession: 002390909

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