+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus

Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus

Nucleic Acids Research 22(2): 208-213

The DNA polymerase gene of African swine fever virus (ASFV) was mapped by marker rescue experiments using a phosphonoacetic acid-resistant mutant and hybridization with an oligonucleotide probe designed from the most conserved motif of family B DNA polymerases. Viral DNA fragments mapping in this region were cloned and sequenced. An open reading frame coding for a 1244 amino acid long peptide with a molecular mass of 142.5 kDa was determined from the sequence. A unique feature of ASFV DNA polymerase is the presence of 13 tandem repeats of the sequence Ala-Gly-Asp-Pro near the carboxyl end of the molecule. Comparison with 30 sequences of alpha-like DNA polymerases of cellular and viral origin showed that ASFV DNA polymerase has all the conserved motifs of family B DNA polymerases. A 3.9 kb transcript was detected by Northern hybridization and the transcription initiation and termination sites were mapped by S1 analysis and primer extension. Late transcription was initiated at a site different from the early transcription initiation site. A 145 kDa protein, consistent with the size of the gene, was identified by an in situ enzyme assay after gel electrophoresis of infected cell extracts.

(PDF emailed within 0-6 h: $19.90)

Accession: 002390921

Download citation: RISBibTeXText

PMID: 8121806

DOI: 10.1093/nar/22.2.208

Related references

Nucleotide sequence of a nucleoside triphosphate phosphohydrolase gene from African swine fever virus. Virus Research 30(1): 63-72, 1993

The DNA polymerase-encoding gene of African swine fever virus: sequence and transcriptional analysis. Gene 136(1/2): 103-110, 1993

Analysis of the complete nucleotide sequence of African swine fever virus. Virology 208(1): 249-278, 1995

Nucleotide sequence of the telomeric region of the African swine fever virus genome. Virology 203(1): 193-196, 1994

Nucleotide sequence and variability of the inverted terminal repetitions of African swine fever virus DNA. Virology 201(1): 152-156, 1994

Nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic African swine fever virus isolate (Malawi LIL20/1). Journal of General Virology 75: 1655-1684, 1994

An African swine fever virus gene with a similarity to eukaryotic RNA polymerase subunit 6. Nucleic Acids Research 21(12): 2940-2940, 1993

Identification of an African swine fever virus gene with similarity to a myeloid differentiation primary response gene and a neurovirulence-associated gene of herpes simplex virus. Journal of Virology 66(9): 5586-5589, 1992

A sequence comparison of the VP72 gene of African swine fever virus. Biochemical Society Transactions 20(1): 30s-30s, 1992

Phylogeographic analysis of African swine fever virus based on the p72 gene sequence. Genetics and Molecular Research 14(2): 4566-4574, 2016

Identification and localization of genetic material of African swine fever virus by autoradiography. Virology 33(1): 173-175, 1967

African swine fever virus thymidylate kinase gene: sequence and transcriptional mapping. Journal of General Virology 74: 1633-1638, 1993

Comparison of the sequence of the gene encoding African swine fever virus attachment protein p12 from field virus isolates and viruses passaged in tissue culture. Journal of Virology 66(6): 3869-3872, 1992