+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Genetic transformation of Populus nigra by Agrobacterium tumefaciens

Genetic transformation of Populus nigra by Agrobacterium tumefaciens

Plant Cell Reports 13(5): 256-261

ISSN/ISBN: 0721-7714

PMID: 24193760

DOI: 10.1007/bf00233315

Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. nigra clones were further analyzed using ß-glucuronidase gene transient expression. The clone Jean Pourtet proved to be more susceptible than the clone San Giorgio. A. tumefaciens strain A281 pKIWI105 proved to be the most virulent. The optimal procedure involved dipping of leaf discs into a bacterial suspension (7×10(8) cells/ml) for 20 min, followed by a 48 h co-cultivation period on semi-solid regeneration medium.Leaf explants were co-cultivated with two disarmed A. tumefaciens strains. Plantlets of San Giorgio were regenerated, tested for ß-glucuronidase activity and rooted on selective medium containing kanamycin. Polymerase chain reaction analysis and Southern blot hybridization confirmed the integration of the neomycin phosphotransferase II gene into the poplar genome.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002391492

Download citation: RISBibTeXText

Related references

Genetic transformation of Populus nigra using Agrobacterium tumefaciens LBA 4404/pBKS-1. Research Report of the Forest Genetics Research Institute 1995(31): 160-166, 1995

Induction of tumors and teratomous shoots and gene transformation of pinus thunbergii and populus koreana x populus nigra var italica by agrobacterium tumefaciens. Seoul National University Journal of Agricultural Sciences 15(1): 1-6, 1990

Transformation of Populus nigra x P. maximowiczii using Agrobacterium tumefaciens vectors. Journal of Korean Forestry Society 87(2): 164-172, 1998

Genetic transformation of Populus deltoides and P.x euramericana clones using Agrobacterium tumefaciens. Plant Cell Tissue and Organ Culture 48(1): 53-61, 1997

Producing herbicide tolerant populus using genetic transformation mediated by Agrobacterium tumefaciens C58: a summary of recent research. NATO ASI series: Series A: Life sciences 1(210): 247-263, 1991

Factors effecting agrobacterium mediated transformation and regeneration of populus nigra x populus maximowiczii. Journal of Korean Forestry Society 79(3): 278-284, 1990

Gene transformation of populus koreana by agrobacterium tumefaciens. Research Report of the Forest Genetics Research Institute (25): 117-122, 1989

Transformation of hybrids Populus tremula X P. alba by Agrobacterium tumefaciens. Somatic cell genetics of woody plants: proceedings of the IUFRO Working Party S204-07 Somatic Cell Genetics held in Grosshansdorf Federal Re ic of Germany 10-13 August 1987 edited by MR Ahuja, 1988

Targeting cells of populus deltoides explants for transformation by agrobacterium tumefaciens. Journal of Cellular Biochemistry Suppl. (13 Part D): 255, 1989

Transformation of Brassica nigra through protoplast cocultivation with Agrobacterium tumefaciens. Current plant science and biotechnology in agriculture: ) 351-352, 1988

Effects of concentration of acetosyringone and Agrobacterium tumefaciens on GUS gene transformation efficiency of Populus. In Vitro Cellular and Developmental Biology Animal 30A(3 Part 2): 67, 1994

Genetic transformation of potato tissue by wild strains of agrobacterium tumefaciens and agrobacterium rhizogenes. Vyestsi Akademii Navuk BSSR Syeryya Biyalahichnykh Navuk (6): 24-28, 1990

An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus). Plant Cell Reports 19(3): 315-320, 2000

Variation in genetic forms of pea Pisum sativum L. in capacity for transformation with different strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes. Genetika Moskva 29(10): 1656-1672, 1993

Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera. Frontiers in Plant Science 7: 296, 2016