EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Identification of the surface polypeptide antigens of the infective larvae of Onchocerca volvulus



Identification of the surface polypeptide antigens of the infective larvae of Onchocerca volvulus



Transactions of the Royal Society of Tropical Medicine and Hygiene 84(5): 696-700



The surface polypeptide antigens of third-stage infective larvae (L3) and adult males of Onchocerca volvulus have been compared after iodogen-catalysed radioiodination, separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in beta-mercaptoethanol followed by autoradiography. L3 surfaces contained polypeptides with apparent molecular weights of 14, 18.5, 26, 34, 51, 68 and 130 kDa, whereas the adults contained 14, 19, 26, 34, 37.5 and 68 kDa components. By immunoprecipitation with patient's sera, only the 14 and 18.5 kDa components were shown to be antigenic on the L3, the other components being unreactive with patients' antibodies. Under similar conditions, 4 of the 6 adult male polypeptides reacted with patients' antisera, confirming their antigenic nature. Lentil lectin and immunosorbent chromatography of the surface components revealed the 18.5 kDa and 68 kDa antigens of L3 and adult males respectively to be glycoproteins. The apparently weak reactivity of L3 surface components with host antibody may be part of an escape mechanism that favours the establishment of O. volvulus in human hosts.

(PDF emailed within 0-6 h: $19.90)

Accession: 002402748

Download citation: RISBibTeXText

PMID: 2278076

DOI: 10.1016/0035-9203(90)90153-6



Related references

Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae. Journal of Clinical Investigation 82(1): 262-269, 1988

Isolation and characterization of expression complementary dna clones encoding antigens of onchocerca volvulus infective larvae. Journal of Clinical Investigation 82(1): 262-269, 1988

Description of an onchocerca volvulus specific dna sequence and the application of the pcr for identification of onchocerca volvulus larvae. Acta Leidensia 59(1-2): 468-469, 1990

Successful vaccination against Onchocerca ochengi infestation in cattle using live Onchocerca volvulus infective larvae. Parasite Immunology 29(3): 113-116, 2007

T-cell-immunoblotting a new approach for the identification of immunodominant antigens of Onchocerca volvulus T-Zell Immunblotting - ein neuer Ansatz zur Identifizierung potentiell schutzvermittelnder Antigene von Onchocerca volvulus. Mitteilungen der Oesterreichischen Gesellschaft fuer Tropenmedizin und Parasitologie, 17: 143-151, 1995

Cryopreservation of infective larvae of Onchocerca volvulus. Journal of Parasitology 79(5): 695-700, 1993

Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use. Molecular and Biochemical Parasitology 20(3): 225-231, 1986

Infective filaria larvae, other than Onchocerca volvulus, in Simulium damnosum. Annals of Tropical Medicine and Parasitology 61(2): 200-205, 1967

Cryopreservation of infective larvae of Onchocerca volvulus (Filarioidea: Onchocercidae). Journal of Parasitology 79(5): 695-700, 1993

A field method for the procurement of infective larvae of Onchocerca volvulus. American Journal of Tropical Medicine and Hygiene 34(4): 731-734, 1985

Morphometric differentiation of Onchocerca volvulus and O. ochengi infective larvae. Transactions of the Royal Society of Tropical Medicine and Hygiene 86(1): 63-65, 1992

Protective immunity against Onchocerca volvulus and O. lienalis infective larvae in mice. Tropical Medicine and Parasitology 45(1): 17-23, 1994

Characterisation of secretory products from the infective third-stage larvae of Onchocerca volvulus. American Journal of Tropical Medicine & Hygiene 61(3 SUPPL ): 446, Sept, 1999