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In vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase from Vicia faba L



In vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase from Vicia faba L



Botanica Acta 105(5): 367-369



The in vitro and in vivo phosphorylation of stomatal phosphoenolpyruvate carboxylase (EC 4.1.1.31) from Vicia faba L. was demonstrated by feeding the concentrated enzyme after 0-70% ammonium sulfate precipitation of guard cell protoplasts with 32P and subsequent analysis of autoradiograms and Western immunoblots, after SDS-PAGE, of protein samples. The in vitro and in vivo results provide evidence for a radioactive labeling of the two stomatal PEPCase bands (112 and 110 kDa).

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Accession: 002405924

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DOI: 10.1111/j.1438-8677.1992.tb00314.x



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