In vitro maturation and fertilization of swamp buffalo (Bubalus bubalis) oocytes
Jainudeen, M.R.; Takahashi, Y.; Nihayah, M.; Kanagawa, H.
Animal Reproduction Science 31(3-4): 205-212
1993
ISSN/ISBN: 0378-4320
DOI: 10.1016/0378-4320(93)90005-c
Accession: 002406315
Cumulus-oocyte complexes collected at slaughter from adult (n = 4) and gonadotropin-treated prepubertal (n = 4) swamp buffaloes were matured in medium 199 with fetal calf serum, antibiotics and hormones (FSH and oestradiol-17 beta ), at 39 degrees C in 5% CO2 and 95% air for 24 h. Freshly collected buffalo semen was diluted with a defined medium containing 5 mg/ml bovine calf serum, and washed by centrifugation.
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