In vitro maturation and fertilization of swamp buffalo (Bubalus bubalis) oocytes

Jainudeen, M.R.; Takahashi, Y.; Nihayah, M.; Kanagawa, H.

Animal Reproduction Science 31(3-4): 205-212


ISSN/ISBN: 0378-4320
DOI: 10.1016/0378-4320(93)90005-c
Accession: 002406315

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Cumulus-oocyte complexes collected at slaughter from adult (n = 4) and gonadotropin-treated prepubertal (n = 4) swamp buffaloes were matured in medium 199 with fetal calf serum, antibiotics and hormones (FSH and oestradiol-17 beta ), at 39 degrees C in 5% CO2 and 95% air for 24 h. Freshly collected buffalo semen was diluted with a defined medium containing 5 mg/ml bovine calf serum, and washed by centrifugation. Cumulus-oocyte complexes were incubated with spermatozoa for 24 h in the medium supplemented with heparin and theophylline. Oocytes were than examined for fertilization after fixation and staining with orcein. For adult and prepubertal animals, the number of cumulus-oocyte complexes harvested were 29 from 35 follicles and 29 from 39 follicles resp., maturation rate was 41% (12 of 29) and 52% (15 of 29), and fertilization rate 21% (6 of 29). Two of 9 inseminated oocytes cultured with oviduct cells developed into morulae 6 days after insemination.