+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling

, : Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling. Virus Research 25(3): 201-211

The protein composition of cellular inclusions induced by the infection of Nicotiana clevelandii with plum pox potyvirus was examined by immunogold electron microscopy of thin sections of leaf tissue. Antisera to CI protein-labelled pinwheel inclusions and laminated aggregates, which were frequently associated with the cell membrane. The NIa and NIb proteins were only associated with crystalline inclusions, mainly detected in the nucleus, but also in the cytoplasm. No structures reacted both with CI and NI antisera using double labelling with gold particles of two different sizes.

Accession: 002416105

DOI: 10.1016/0168-1702(92)90134-U

Download PDF Full Text: Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Martin, M.T.; Gelie, B., 1997: Non-structural plum pox potyvirus proteins detected by immunogold labelling. Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclu...

Grigoriev, V.B.; Escaig-Haye, F.; Sharova, N.K.; Rudneva, I.A.; Simonov, V.; Kazennova, E.K.; Kush, A.A.; Klimenko, S.M.; Buckrinskaya, A.; Fournier, J.G., 1992: Localization by immunogold labelling of HIV-1 structural proteins on Lowicryl embedded HIV-1 infected cell ultrathin sections. Using several HIV-1-specific antibodies and the immunogold labelling technique, we have detected and localized distinct viral proteins on ultrathin sections of HIV-1 infected cells embedded in the Lowicryl K4M resin. Monoclonal antibodies (MAbs) a...

Hills G.J.; Plaskitt K.A.; Young N.D.; Dunigan D.D.; Watts J.W.; Wilson T.M.A.; Zaitlin M., 1987: Immunogold localization of the intracellular sites of structural and nonstructural tobacco mosaic virus proteins. Antibodies raised against the 126K nonstructural protein (replicase) encoded by tobacco mosaic virus (TMV) RNA or the viral coat protein have been used to localize these proteins within virus-infected tobacco leaf cells by an immunogold labeling t...

Cambra, M.; Asensio, M.; Gorris, M.T.; Perez, E.; Camarasa, E.; Garcia, J.A.; Moya, J.J.; Lopez Abella, D.; Vela, C.; Sanz, A., 1994: Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. Eleven monoclonal antibodies specific to plum pox potyvirus (PPV) coat protein were obtained by hybridoma technology from Spanish PPV isolates. In addition, two monoclonal antibodies specific for PPV cylindrical inclusions (CIP non-structural prot...

Riedel, D.; Lesemann, D.E.; Maiss, E., 1998: Ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus. Antisera to the bacterially expressed nonstructural proteins (NSP) HC-Pro, CI, NIa, and NIb and the coat protein (CP) of plum pox potyvirus (PPV) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electr...

Adamolle, C.; Quiot, L.; Bousalem, M.; Boeglin, M.; Quiot, J.B., 1994: Use of antisera to non-structural proteins in the study of plum pox potyvirus. A possible approach for the detection and characterization of strains of plum pox potyvirus (PPV) is the use of antisera to non-structural proteins, such as the cylindrical inclusion protein (CIP) and the nuclear inclusion 'a' protein (N...

Semeshin, V.F.; Shloma, V.V.; Andreeva, E.N.; Saumweber, H.; Zhimulev, I.F., 2003: Use of immunogold labelling technique for immunoelectron microscope localization of proteins in Drosophila polytene chromosomes. Using gold labeled antibodies, we developed and tested an immunoelectron microscope (IEM) method for detection of protein localization in Drosophila melanogaster polytene chromosomes. This method is based on procedures widely used for indirect imm...

Morin, X.; Soll, J., 1997: Immunogold labelling of cryosectioned pea chloroplasts and initial localization of the proteins associated with the protein import machinery. The electron-microscopic technique for immunogold labelling of thawed cryosectioned material (K.T. Tokuyasu, 1989, Histochem J 21: 163-171) has been adapted for use with isolated chloroplasts. Percoll-purified pea (Pisum Sativum L. cv Feltham Firs...

Adler, K.; Arkona, C.; Manteuffel, R.; Suess, K.H.inz, 1993: Electron-microscopical localization of chloroplast proteins by immunogold labelling on cryo-embedded spinach leaves. Cell Biology International 17(2): 213-220

Escaig-Haye, F.; Grigoriev, V.; Sharova, I.; Rudneva, V.; Buckrinskaya, A.; Fournier, J.G., 1992: Ultrastructural localization of HIV-1 RNA and core proteins. Simultaneous visualization using double immunogold labelling after in situ hybridization and immunocytochemistry. The analysis of human immunodeficiency virus type 1 (HIV-1) RNA sequences in CEM and Jurkat lymphoid cells infected with the virus has been performed at the subcellular level. Using a biotinylated DNA probe specific for HIV-1, virus RNA sequences...