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Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling


, : Intracellular localization of three non-structural plum pox potyvirus proteins by immunogold labelling. Virus Research 25(3): 201-211

The protein composition of cellular inclusions induced by the infection of Nicotiana clevelandii with plum pox potyvirus was examined by immunogold electron microscopy of thin sections of leaf tissue. Antisera to CI protein-labelled pinwheel inclusions and laminated aggregates, which were frequently associated with the cell membrane. The NIa and NIb proteins were only associated with crystalline inclusions, mainly detected in the nucleus, but also in the cytoplasm. No structures reacted both with CI and NI antisera using double labelling with gold particles of two different sizes.

Accession: 002416105

DOI: 10.1016/0168-1702(92)90134-U

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Related references

Martin, M.T.; Gelie, B., 1997: Non-structural plum pox potyvirus proteins detected by immunogold labelling. Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclu...

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