EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Intracerebroventricularly administered corticotropin-releasing factor releases somatostatin through a cholinergic, vagal pathway in freely fed rats






Acta Physiologica Scandinavica 151(2): 241-248

Intracerebroventricularly administered corticotropin-releasing factor releases somatostatin through a cholinergic, vagal pathway in freely fed rats

The aim of this study was to investigate whether corticotropin-releasing factor influences the plasma levels of somatostatin, gastrin or cholecystokinin when administered intracerebroventricularly to rats, and if such an effect could be vagally mediated, and dependent on the animals feeding states. Anaesthetized, freely fed rats were given 5 mu-l intracerebroventricular injections of corticotropin-releasing factor in four doses; 10 pmol-1.28 nmol. Immediately following death, trunk blood was collected for subsequent peptide analysis with radioimmunoassay (RIA). The three higher doses of corticotropin-releasing factor elevated the plasma levels of somatostatin (P lt 0.01) after 20 min but left the plasma levels of gastrin and cholecystokinin unchanged. Intraperitoneal injections of 60 and 320 pmol of corticotropin-releasing factor did not influence the somatostatin levels. Further, intracerebroventricular injections of 60 pmol of corticotropin-releasing factor produced a peak increase in somatostatin after 20 min (P lt 0.01). After 60 min the somatostatin levels were still increased (P lt 0.05). Gastrin and cholecystokinin remained unaltered at these timepoints. Intracerebroventricular administration of 10 nmol of alpha-helical corticotropin-releasing factor 9-41 attenuated the basal levels of somatostatin and blocked the corticotropin-releasing factor-induced rise in somatostatin. Bilateral truncal vagotomy, as well as pretreatment with atropine (0.05 mg kg-1, subcutaneously) abolished the effects of corticotropin-releasing factor on somatostatin. In animals which were food-deprived for 24 h, corticotropin-releasing factor did not influence somatostatin, gastrin or cholecystokinin. Pretreatment with cholecystokinin did not potentiate corticotropin-releasing factor-induced somatostatin release in food-deprived rats. These findings suggest that corticotropin-releasing factor acting within the central nervous system may regulate gastrointestinal functions partially through a cholinergic, vagally mediated release of somatostatin in freely fed, but not in food-deprived rats.

Accession: 002416121

PMID: 7942060

DOI: 10.1111/j.1748-1716.1994.tb09743.x

Download PDF Full Text: Intracerebroventricularly administered corticotropin-releasing factor releases somatostatin through a cholinergic, vagal pathway in freely fed rats



Related references

Intracerebroventricularly administered corticotropin-releasing factor inhibits food intake and produces anxiety-like behaviour at very low doses in mice. Diabetes, Obesity & Metabolism 1(5): 281-284, 2001

Influence of intracerebroventricularly administered ovine corticotropin releasing factor crf 1 41 on passive avoidance behavior lack of effect on monoamine contents of limbic brain areas. Neuroscience Letters (SUPPL 18): S340, 1984

Sympatho-adrenal medullary functions in response to intracerebroventricularly injected corticotropin-releasing factor in anesthetized rats. Brain Research 367(1-2): 250-257, 1986

Intracerebroventricularly administered corticotropin releasing hormone potently affects behavior without altering antinociceptive responding. Life Sciences 39(5): 433-442, 1986

Intracerebroventricularly administered lipopolysaccharide enhances spike-wave discharges in freely moving WAG/Rij rats. Brain Research Bulletin 85(6): 410-416, 2012

A comparison of synthetic human and rat ANP administered intracerebroventricularly in freely moving normotensive and hypertensive rats. Journal of Cardiovascular Pharmacology 13 Suppl 6: S27-S30, 1989

Improved microdialysis measurements of corticotropin-releasing factor release from limbic brain structures of freely moving rats. Society for Neuroscience Abstracts 19(1-3): 171, 1993

Corticotropin-releasing factor injected intravenously increases gastric vagal afferent discharge in rats. Gastroenterology 108(4 SUPPL ): A631, 1995

Corticotropin-releasing factor stimulates catecholamine release in hypothalamus and prefrontal cortex in freely moving rats as assessed by microdialysis. Journal Of Neurochemistry. 60(2): 602-612, 1993

Changes of immunoreactive somatostatin, neuropeptide Y, and corticotropin-releasing factor (CRF) in the brain of spontaneously epileptic rats (SER). Japanese Journal of Psychiatry and Neurology 46(2): 531-533, 1992