+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii

, : Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii. Journal of Bacteriology 175(9): 2516-2522

The spirochete Borrelia hermsii, a relapsing fever agent, evades the host's immune response through multiphasic antigenic variation. Antigen switching results from sequential expression of genes for serotype-specific outer membrane proteins known as variable major proteins (Vmp's); of the 25 serotypes that have been identified for the HS1 strain, serotypes 7 and 21 have been studied in greatest detail. In the present study, an atypical variant was predominant in the relapse from a serotype 21 infection in mice; relapse cells were bound by monoclonal antibodies specific for Vmp21 as well as antibodies specific for Vmp7. In Western blots (immunoblots), the variant had a single Vmp that was reactive with monoclonal antibodies representing both serotypes. The gene encoding this Vmp, vmp7/21, was cloned and characterized by restriction mapping and sequence analysis to determine the likely recombination event. Whereas the 5' end of vmp7/21 was identical to that of vmp21, its 3' end and flanking sequences were identical to the 3' end of vmp7. Unlike other vmp genes examined thus far, the vmp7/21 gene existed only in an expressed form; a silent, storage form of the gene was not detected. We conclude that the vmp7/21 gene was created by an intragenic recombination between the formerly expressed vmp21 gene and a silent vmp7 gene. This finding suggests that the lack of cross-reactivity between variants, which is usually observed, results from immunoselection against variants possessing chimeric Vmp's rather than from a switching mechanism that excludes partial gene replacements.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002416151

PMID: 7683020

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ono, T.; Obara, Y., 1989: Karyotype and NORs of a female Asian free-tailed bat, Tadarida insignis (Molossidae: Chiroptera). CIS (Chromosome Information Service): 17-19 No. 46

Semeniuk, G.; Wallin, J. R., 1947: The influence of climate on four leaf parasites of Zea mays in Guatemala. Samples of maize obtained from various localities in Guatemala were exposed at 6 stations, ranging from sea level to over 8000 ft. altitude, to natural infection by Puccinia sorghi, Helminthosporium turcicum, Angiopsora zeae, and Phyllachora zeae.

Ku, Yoe Ray; Lin, Yaa Tzy; Lin, Jer Huei; Wen, Kuo Ching; Liao, Chun Heng, 1998: Determination of parishin, parishin B and parishin C in traditional Chinese medicinal formulas by micellar electrokinetic capillary chromatography. Micellar electrokinetic capillary chromatographic methods were established for the determination of parishin, parishin B and parishin C in two kinds of the traditional Chinese medicinal formulas including Gastrodiae Rhizoma: Tsan-Siang-Tian-Ma-Tan...

Kolodrubskii, V.; Savich, V., 1988: Theoretical problems of forming an accounting system linking the branches of the agroindustrial sector. The functional efficiency of the agroindustrial sector depends on the suitability of its accounting system. In the USSR this must consist of three levels: the farm or enterprise accounting system, the accounting system at the level of the agroindu...

Yagishita S.; Itoh Y.; Nakano T.; Amano N.; Yokoi S.; Hasegawa O.; Tanaka T., 1983: Pleomorphic intra neuronal poly glucosan bodies mainly restricted to the pallidum a case. Acta Neuropathologica 62(1-2): 159-163

Ji ChengYe, 2005: Report on childhood obesity in China: body mass index reference for screening overweight and obesity in Chinese school-age children. Purpose: To establish and propose a national body mass index (BMI) reference for screening overweight and obesity in Chinese school children and adolescents. Methods: The 2000 Chinese National Survey on Students Constitution and Health data, obtai...

Vinceti, M.; Bergomi, M.; Borciani, N.; Serra, L.; Vivoli, G., 1999: Rising melanoma incidence in an Italian community from 1986 to 1997. We examined the incidence of primary invasive melanoma in the municipality of Reggio Emilia, northern Italy, in the period from 1986 to 1997. We identified 169 cases, five of which were intraocular. After adjustment for confounders, the risk of ha...

PINCOCK, T.A., 1964: Alcoholism In Tuberculous Patients. The incidence of alcoholism among patients suffering from tuberculosis in the Tuberculosis Hospital in Winnipeg was determined, and attention is drawn to the need to treat tuberculosis and alcoholism concurrently. In all, 306 patients with tubercu...

Beregovoi, V. E., 1962: Contribution to the study of Latrodectus pattidus O. Cambr. subsp. pavlovskyi Charit. Zoologicheskii Zhurnal, 41: 528-538

Bhattacharya, T. K.; Gandhi, R. S., 2005: Principal components versus multiple regression analysis to predict lifetime production of Karan Fries cattle. Data on 792 Karan Fries dairy cows between 1970-1995 in Karnal, Haryana, India, were analysed to formulate principal component scores (PCS) based on phenotypic correlations among early lactation traits. Out of 14 early lactation traits, 7 each per...