EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Introduction of a herbicide resistant gene into an apple root-stock with Agrobacterium tumefaciens


, : Introduction of a herbicide resistant gene into an apple root-stock with Agrobacterium tumefaciens. Bulletin of the Faculty of Agriculture, Hirosaki University ( 57): 1-8

Leaf segments of the stock Malus prunifolia var. ringo were inoculated with a strain of the bacterium into which a gene for resistance to the herbicide bialaphos (bar, encoding phosphinothricin acetyltransferase) was introduced as a binary vector. The segments produced callus on selection medium containing the herbicide, and regenerated shoots were obtained from the callus. Presence of the gene in the host genome was confirmed by PCR analysis.


Accession: 002416392

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Igarashi, M.; Ogasawara, H.; Hatsuyama, Y.; Saito, A.; Suzuki, M., 2002: Introduction of rolC into Marubakaidou apple rootstock via Agrobacterium tumefaciens. We attempted to produce new dwarf rootstock cultivars with high rooting ability from cuttings by introducing the rolC gene derived from Agrobacterium rhizogenes. Southern blot analysis demonstrated that one to three copies of the transgene had bee...

Otani, M.; Wakita, Y.; Shimada, T., 2003: Production of herbicide-resistant sweetpotato Lam plants by Agrobacterium tumefaciens-mediated transformation. Breeding Science 53(2): 145-148, June

Zang Ning; Zhang MeiYan; Zhai Hong; L.X.eQin; Wang YuPing; Liu QingChang, 2008: Generation of transgenic herbicide-resistant sweetpotato plants by Agrobacterium tumefaciens-mediated transformation. Generation of transgenic sweetpotato plants expressing bar gene for herbicide resistance was achieved using Agrobacterium tumefaciens-mediated transformation and sweetpotato (Ipomoea batatas (L.) Lam.) cv. Lizixiang embryogenic suspension cultures...

Choi, H.-Jin-; Chandrasekhar, T.-; Lee, H.-Yeon-; Kim, K.-Moon, 2007: Production of herbicide-resistant transgenic sweet potato plants through Agrobacterium tumefaciens method. Transgenic herbicide-resistant sweet potato plants [Ipomoea batatas (L.) Lam.] were produced through Agrobacterium-mediated transformation system. Embryogenic calli derived from shoot apical meristems were infected with Agrobacterium tumefaciens s...

Eady, C.; Davis, S.; Farrant, J.; Reader, J.; Kenel, F., 2003: Agrobacterium tumefaciens-mediated transformation and regeneration of herbicide resistant onion (Allium cepa) plants. Transgenic onion plants (Allium cepa) tolerant to herbicides containing active ingredients glyphosate and phosphinothricin were recovered from immature embryos of open pollinated and hybrid parent onion lines at a maximum transformation frequency...

Otani, M.; Wakita, Y.; Shimada, T., 2003: Production of herbicide-resistant sweetpotato (Ipomoea batatas (L.) Lam.) plants by Agrobacterium tumefaciens-mediated transformation. The expression of the bar gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter or the modified CaMV35S promoter, E12 Omega promoter and the subsequent herbicide tolerance of the transgenic sweet potato plants are discussed.

Ribas, A.-Ferreira; Kobayashi, A.-Kenji; Pereira, L.-Filipe-Protasio; Vieira, L.-Gonzaga-Esteves, 2006: Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation. Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacteriurn tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and...

Rebandel, Z.W.erszyllowski, J.G.uca, Z., 1977: Evaluation of the utility of rootstocks infected with crown gall (Agrobacterium tumefaciens (Smith et Town) Conn. in apple nursery stock production

Viss, W.; Pitrak, J.H.mann, J.C.ok, M.D.iver, J.R.am, W., 2003: Crown-gall-resistant transgenic apple trees that silence Agrobacterium tumefaciens oncogenes. Crown gall disease is an economically significant problem in fruit and nut orchards, vineyards, and nurseries worldwide. Tumors on stems and leaves result from excessive production of the phytohormones auxin and cytokinin in plant cells geneticall...

Anonymous, 2001: Production of transgenic rice plants resistant to yellow stem borer and herbicide in two Vietnamese varieties via Agrobacterium tumefaciens