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Introduction of a herbicide resistant gene into an apple root-stock with Agrobacterium tumefaciens

, : Introduction of a herbicide resistant gene into an apple root-stock with Agrobacterium tumefaciens. Bulletin of the Faculty of Agriculture, Hirosaki University ( 57): 1-8

Leaf segments of the stock Malus prunifolia var. ringo were inoculated with a strain of the bacterium into which a gene for resistance to the herbicide bialaphos (bar, encoding phosphinothricin acetyltransferase) was introduced as a binary vector. The segments produced callus on selection medium containing the herbicide, and regenerated shoots were obtained from the callus. Presence of the gene in the host genome was confirmed by PCR analysis.

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