+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Introduction of hygromycin resistance in Lotus spp. through Agrobacterium rhizogenes transformation

Transgenic Research 2(6): 330-335

Introduction of hygromycin resistance in Lotus spp. through Agrobacterium rhizogenes transformation

Lotus corniculatus and L. tenuis were transformed with the Agrobacterium rhizogenes binary vector LBA9402 carrying the wild-type Ri plasmid 185S and plasmid AGS12S (kanamycin resistant) containing, between A. tumefaciens borders, the hygromycin phosphotransferase gene under the control of the nos promoter. Transgenic plants recovered from both species were tested for the ability of leaf-derived calluses to grow in a hygromycin-supplemented medium.

Accession: 002416417

Related references

Transformation of Medicago arborea L. with an Agrobacterium rhizogenes binary vector carrying the hygromycin resistance gene. Plant Cell Reports 10(6-7): 300-303, 1991

Improvement of a method for the transformation of potato using Agrobacterium rhizogenes: application to the introduction of Phthorimaea operculella resistance into variety Fanette. Proceedings of the Joint Conference of the EAPR Breeding and Varietal Assessment Section and the EUCARPIA Potato Section, Landerneau, France, 12-17 January 1992: 87-91, 1992

Rapid transformation of Lotus corniculatus L by Agrobacterium rhizogenes Regeneration via shoot organogenesis. Bulgarian Journal of Plant Physiology (Special Issue): 66, 1998

Agrobacterium rhizogenes as a vector for transforming higher plants. Application in Lotus corniculatus transformation. Methods in Molecular Biology 49: 49-61, 1995

Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics. Bmc Plant Biology 9(): 78-78, 2009

Optimization of potato genetic transformation by Agrobacterium tumefaciens using hygromycin resistance as selective marker. Biotechnologie, Agronomie, Societe et Environnement 7(3/4): 183-188, 2003

Transformation with Agrobacterium rhizogenes induces nematode resistance in Raphanus sativus roots. Nematology 2(7): 761, 2000

Evaluation of agrobacterium-mediated transformation of Agaricus bisporus using a range of promoters linked to hygromycin resistance. Molecular Biotechnology 32(2): 129-138, 2006

Study of the variability of pea response to transformation with Agrobacterium tumefaciens and agrobacterium rhizogenes strains. Genetika 29(10): 1656-1672, 1993

Transformation of brassica napus l. oilseed rape using agrobacterium tumefaciens and agrobacterium rhizogenes a comparison. Plant Science (Shannon) 70(1): 91-100, 1990