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Introduction of hygromycin resistance in Lotus spp. through Agrobacterium rhizogenes transformation

, : Introduction of hygromycin resistance in Lotus spp. through Agrobacterium rhizogenes transformation. Transgenic Research 2(6): 330-335

Lotus corniculatus and L. tenuis were transformed with the Agrobacterium rhizogenes binary vector LBA9402 carrying the wild-type Ri plasmid 185S and plasmid AGS12S (kanamycin resistant) containing, between A. tumefaciens borders, the hygromycin phosphotransferase gene under the control of the nos promoter. Transgenic plants recovered from both species were tested for the ability of leaf-derived calluses to grow in a hygromycin-supplemented medium.

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