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Intron sequence directs RNA editing of the glutamate receptor subunit GluR2 coding sequence


, : Intron sequence directs RNA editing of the glutamate receptor subunit GluR2 coding sequence. Proceedings of the National Academy of Sciences of the United States of America 91(22): 10270-4

The Ca2+ permeability and the rectifying properties of the glutamate receptors assembled from the subunits GluR1-GluR4 depend upon a critical Arg in the GluR2 subunit located in a domain that has been proposed to span the membrane. The GluR2 subunit gene encodes a Gln (CAG) at this position, whereas the mRNA is edited so that it encodes an Arg (CGG) at this position [Sommer, B., Kohler, M., Sprengel, R. & Seeburg, P. H. (1991) Cell 67, 11-20]. The editing process is specific since only the GluR2 subunit RNA is edited even though the GluR1, GluR3, and GluR4 RNAs have a similar sequence. We show that this selective RNA editing depends upon a critical intron sequence in the GluR2 gene. This critical intron sequence is sufficient to cause editing of the GluR3 subunit exon in a chimera minigene constructed so that the GluR3 exon is placed upstream to the GluR2 intron sequence. Transfections of a neuronal cell line, N2a, with minigene constructs encoding different fragments of the GluR2 gene demonstrate that the 5' part of the 3' intron is essential for editing. Part of the exon and this critical intron sequence contains an inverted repeat that can fold into a structure consisting of three helical elements. Similar conclusions were reached by Higuchi, M., Single, F. n., Köhler, M., Sommer, B., Sprengel, R. & Seeburg, P. H. [(1993) Cell 75, 1361-1370]. These experiments demonstrate that the low Ca2+ permeability of the ionotropic non-N-methyl-D-aspartate glutamate receptors depends upon RNA editing, which requires a sequence in an intron 3' to the exon.

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Accession: 002416535

PMID: 7937939

DOI: 10.2307/2366009

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Simpson, I. G., 1972: Two systems of beef production on arable farms. An economic study on farms in the East of England, 1968 to 1970. The report covers an investigation into the economics of two specific systems of beef production on lowland arable farms. The two systems of ' single suckling ' and ' 18 month beef', the latter using autumn born calves. The res...

Takiguchi, M.; Fujinaga, T.; Naiki, M.; Mizuno, S.; Otomo, K., 1990: Isolation, characterization, and quantitative analysis of C-reactive protein from horses. C-reactive protein (CRP) was isolated from equine serum by use of calcium-dependent affinity chromatography conjugated pneumococcal C-polysaccharide, anion exchange chromatography, and gel filtration. It was identified as genuine CRP by its immuno...

Lee, C. D.; Penrose, L.S., 1946: A contribution to the genetics of hair colour in man. Following the technique of Arnow the writers have confirmed that pigment could be obtained by this method from red hair, but they also obtained apparently identical pigment from all types of hair. The conc. of this pigment seems to be genetically...

Heyrovsky, L., 1936: Dritter Beitrag zur Kenntnis der Gruppe Lepturini (Col. Ceraml).). Acta Societatis Entomologicae Cechoslovenicae, 33: 52-57

Peterson, B. J.; Hobbie, J. E.; Haney, J. F., 1978: Daphnia grazing on natural bacteria. Daphnia (10-16) were placed in small vials filled with natural water from a lake and incubated for 0.5-2.0 h. The small (ca. 0.10 .mu.3) bacteria were counted on Nuclepore filters by an Acridine Orange direct count method with epifluorescence. Fil...

Mozzarelli, A.; Bettati, S.; Pucci, A.M.; Burkhard, P.; Cook, P.F., 1998: Catalytic competence of O-acetylserine sulfhydrylase in the crystal probed by polarized absorption microspectrophotometry. The reactions of the pyridoxal 5-phosphate-dependent enzyme O-acetylserine sulfhydrylase with the substrate O-acetyl-L-serine and substrate analogs have been investigated in the crystalline state by single-crystal polarized absorption microspectro...

Nikulasson, S. T.; Fogt, F.; Gibbons, D.; Hallgrimsson, J.; Holden, J.; Alder, S. L.; O'brien, M. J., 1996: Comparison of topoisomerase II-alpha and MIB1 expression in benign, dysplastic and malignant gastric mucosal biopsies. Modern Pathology 9(1): 63A

Koeze, T.H.; Simpson, B.A.; Watkins, E.S., 1984: Diagnosis and repair of malfunctions of implanted central nervous system stimulators. Neurostimulator malfunctions must be located and repaired if patients are to receive maximum benefit from CNS stimulation [for the relief of pain, intractable epilepsy and certain movement disorders]. The problems encountered with the stimulators...

Nicolaidis, S.; Gerozissis, K.; Orosco, M., 1994: Hypothalamic dynamic insulin changes in Wistar rats, in relation to meals and to the macronutrient composition. Society for Neuroscience Abstracts 20(1-2): 1601

Ipatov, V. S.; Gerasimenko, G. G.; Kirikova, L. A.; Samoilov, Yu, I.; Trofimets, V. I., 1995: Autogenic successions in the lichen-moss pine forest. 1. The phytocoenological analysis of species composition. The analysis of species composition at different phases of autogenic successions is the part of monographic investigation of the lichen-moss pine forests. Using ecological and biological characteristics of these species and interspecific associati...