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Intronic androgen response elements of prostatic binding protein genes

, : Intronic androgen response elements of prostatic binding protein genes. Biochemical & Biophysical Research Communications 191(2): 688-694

Transcription of the genes that code for the different components of Prostatic Binding Protein is dependent on androgens. DNA-cellulose competition assays have demonstrated the presence of regions with affinity for the androgen receptor located immediately upstream and in the first intron of these genes. DNase I foodtprinting in vitro now reveals the exact binding site of a receptor dimer in the first intron of the C3(1), C3(2) and C1 genes. Gene transfer experiments resulted in the description of functional androgen response elements in the C3 genes. The C1 intronic binding site, however, has a much weaker affinity for the DNA-binding domain of the androgen receptor, a characteristic that might explain its inability to function as an androgen response element in chimeric gene constructs.

Accession: 002416539

PMID: 8461022

DOI: 10.1006/bbrc.1993.1272

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Related references

Claessens F.; Rombauts W.; Davies P.; Rushmere N.; Peeters B.; Celis L.; Heyns W.; Verhoeven G., 1989: Characterization of androgen response elements in prostatic binding protein genes. Journal of Endocrinology 123(SUPPL): 75

Claessens, F.; Celis, L.; D.V.s, P.; Heyns, W.; Verhoeven, G.; Peeters, B.; Rombauts, W., 1993: Functional androgen response elements in the genes coding for prostatic binding protein. Annals of the New York Academy of Sciences 684: 199-201

Rushmere, N.K.; Claessens, F.; Peeters, B.; Rombauts, W.; Davies, P., 1990: Intronic steroid response elements in prostate binding protein genes. Biochemical Society Transactions 18(4): 560-561

Gregory, C.W.; Avellar, M.C.W.; Power, S.G.A.; Marschke, K.B.; Tan, J.A.; Wilson, E.M.; French, F.S., 1995: Localization of androgen-regulated DNA/protein interactions in the intron 1 androgen response elements of the 20 kDa protein and C3 subunit genes. Journal of Andrology 0(SUPPL ): P36

Gregory, C.W.; Power, S.G.A.; Avellar, M.C.W.; Marschke, K.B.; Tan, J.A.; Wilson, E.M.; French, F.S., 1994: Androgen-induced DNA/protein interactions localized to androgen response elements in intron 1 of the 20 kDa and C3 genes. Molecular Biology of the Cell 5(SUPPL ): 20A

Claessens, F.; Rushmere, N.K.; Davies, P.; Celis, L.; Peeters, B.; Rombauts, W.A., 1990: Sequence-specific binding of androgen-receptor complexes to prostatic binding protein genes. Prostatic binding protein is a complex glycoprotein comprising three components, C1, C2 and C3, organized into two different heterodimers (C1-C3 and C2-C3). The rat ventral prostate genes encoding all three constituent polypeptides are expressed u...

Page M.J.; Parker M.G., 1982: Effect of androgen on the transcription of rat prostatic binding protein genes. The mechanisms whereby androgens modulate mRNA levels for the C1, C2 and C3 prostatic binding protein genes in the rat ventral prostate were investigated. Transcription rates for the 3 genes were lowered 2 to 3-fold upon castration but increased a...

Comuzzi, B.; Lambrinidis, L.; Rogatsch, H.; Godoy-Tundidor, S.; Knezevic, N.; Krhen, I.; Marekovic, Z.; Bartsch, G.; Klocker, H.; Hobisch, A.; Culig, Z., 2003: The transcriptional co-activator cAMP response element-binding protein-binding protein is expressed in prostate cancer and enhances androgen- and anti-androgen-induced androgen receptor function. Progression of human prostate cancer toward therapy resistance occurs in the presence of wild-type or mutated androgen receptors (ARs) that, in some cases, exhibit aberrant activation by various steroid hormones and anti-androgens. The AR associat...

Claessens, F.; Dirckx, L.; Delaey, B.; Decourt, J.L.; Hemschoote, K.; Peeters, B.; Rombauts, W., 1989: The androgen-dependent rat prostatic binding protein: comparison of the sequences in the 5' part and upstream region of the C1 and C2 genes and analysis of their transcripts. The complete gene encoding the polypeptide C1 of the complex androgen-controlled prostatic binding protein was isolated from a rat genomic library. A new genomic fragment (C2B) containing only the 5' part of a C2-related gene was also purifie...

Claessens, F.; Celis, L.; Peeters, B.; Heyns, W.; Verhoeven, G.; Rombauts, W., 1989: Functional characterization of an androgen response element in the first intron of the C3(1) gene of prostatic binding protein. We demonstrate that the 204 bp intronic gene fragment of C3(1), which has a specific in vitro affinity for the androgen receptor, is able to confer androgen responsiveness to a heterologous promoter. This characteristic is completely destroyed by...