+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Investigating the relationship between aluminium toxicity, root growth and root-generated ion currents

Developments in plant and soil sciences5(45): 769-778
Investigating the relationship between aluminium toxicity, root growth and root-generated ion currents
One of the primary initial symptoms of Al toxicity is inhibition of root growth. Also, there is a growing body of evidence indicating that natural electric currents play a major role in the differentiation and growth of cells, tissues, and organs, including apical growth of roots. In this study, we are using a highly sensitive extracellular vibrating microelectrode system to map the ion current patterns surrounding growing root apices of wheat cultivars (Triticum aestivum L. aAtlas 66' and aScout 66') which differ in Al tolerance. When the seedlings were grown in a solution consisting of 0.6 mol m(-3)CaCl2 (pH 4.50), a positive current (1-2 microAmps cm(-2)) was measured entering the root cap, apical meristem, and region just behind the apical meristem in both aScout' and aAtlas'. Exposure of the roots to 10 mmol m(-3) AlCl3 elicited a significant inhibition of this inward current in aScout' after approx. 3 hours exposure to Al. This same level of Al inhibited root growth in aScout'; the time course for Al-induced inhibition of root growth and ion currents were nearly identical. In aAtlas', 10 mmol m(-3) Al had no effect on root-generated currents or root growth. A higher level of Al (50 mmol m(-3) AlCl3), that had some inhibitory effect on aAtlas' root growth, was also used. This level of Al more dramatically inhibited aScout' root growth and the ion currents associated with the root apex. However, an exposure period of about 3 hrs was still necessary to observe an inhibition of growth and root currents. This same Al level caused a moderate inhibition of both aAtlas' root growth and inward current with similar time courses. The striking similarities between the time course of Al-induced inhibition of root growth and root-associated currents strongly suggest that the ion transport processes resulting in inward current at the root apex are involved in root growth, and Al-induced alteration of these transport processes may be an important aspect of the early stages of Al toxicity.

Accession: 002416687

Related references

Investigations into the relationship between aluminum toxicity root growth and ion currents associated with the growing root. Plant Physiology (Rockville) 93(1 SUPPL): 101, 1990

Aluminium toxicity in roots. Correlation among ionic currents, ion fluxes, and root elongation in aluminium-sensitive and aluminium-tolerant wheat cultivars. Plant Physiology 99(3): 1193-1200, 1992

Correlation between root generated ionic currents ph fusicoccin iaa and growth of the primary root of zea mays. Plant Physiology (Rockville) 89(4): 1198-1206, 1989

Correlation between Root-Generated Ionic Currents, pH, Fusicoccin, Indoleacetic Acid, and Growth of the Primary Root of Zea mays. Plant Physiology 89(4): 1198-1206, 1989

Aluminium toxicity in rye (Secale cereale): root growth and dynamics of cytoplasmic Ca2+ in intact root tips. Annals of Botany 89(2): 241-244, 2002

Fast root growth responses, root exudates, and internal detoxification as clues to the mechanisms of aluminium toxicity and resistance: a review. Environmental and Experimental Botany 48(1): 75-92, 2002

Aluminium toxicity and regeneration on the root cap. Preliminary evidence for a Golgi apparatus derived morphogen in the primary root of Zea mays. South African Journal of Botany 51(5): 363-370, 1985

Aluminium toxicity and regeneration of the root cap: preliminary evidence for a Golgi apparatus derived morphogen in the primary root of Zea mays. South African journal of botany = Suid Afrikaanse tydskrif vir plantkunde 51(5): 363-370, 1985

Relationship between root cation-exchange capacity and aluminium toxicity in two pasture grasses. Pesquisa Agropecuaria Brasileira 19(5): 631-637, 1984

Relationship between the root cation exchange capacity of sorghum and the tolerance to aluminium toxicity. Agronomia Tropical Maracay 36(4-6): 115-128, 1986