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Investigation into the kinetic properties of immobilized lignin peroxidases


, : Investigation into the kinetic properties of immobilized lignin peroxidases. Journal of Biotechnology 35(1): 77-85

Mixtures of lignin peroxidase isozymes were immobilized onto controlled-pore glass (CPG) beads through three different functional groups (free amino groups, free carboxylate groups, or the aldehyde groups of the metaperiodate-oxidized carbohydrate portions of the proteins) and catalytic properties of the immobilized proteins were determined. Some loss of activity occurred in all immobilized samples, but relatively high activities were maintained with enzymes immobilized via the modified carbohydrate moieties. The effectiveness of using the immobilized enzymes to treat anthracene and pulp mill bleach plant effluent was tested. Bleach plant effluent precipitated onto the aminopropyl-CPG beads inactivating the enzymes, probably due to reactions with free amino groups. In contrast, anthracene was rapidly and completely degraded by the immobilized lignin peroxidases.

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Accession: 002416720

DOI: 10.1016/0168-1656(94)90191-0

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Arzani, L., 1975: Experiments on the interactions between plant density, irrigation and nitrogen fertilization in relation to forage (silage) and grain yields of some maize varieties. Four maize hybrids were studied with and without irrigation at five plant densities and three nitrogen fertilizer rates for forage yield and two of them alone for grain yield. There were no significant differences between the varieties for forage...

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Hassan H.T.; Rees J.K.H., 1990: Relation between age and blast cell differentiation in acute myeloid leukemia patients. The relation between age and the cytological and cytochemical features of blast cell differentiation in 211 patients with primary acute myeloid leukaemia (AML) was investigated. Five cytological and cytochemical features of blast cell differentiat...

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Volkova L.V., 1980: New pathways of transmission of genetic information in mammalian cells in vitro. Biologicheskie Nauki (Moscow) (4): 38-49

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