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Investigation of chloroplast DNA heteroplasmy in Medicago sativa L. using cultured tissue


, : Investigation of chloroplast DNA heteroplasmy in Medicago sativa L. using cultured tissue. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 86(1): 65-70

Medicago sativa L. cv Regen S is heteroplasmic for chloroplast DNA (cpDNA). Previous analyses of regenerated plants have shown a predominance of one of the cpDNAs which we have designated type A (the other we have designated type B). Studies of the replication of the two cpDNAs in tissue culture were carried out using leaflet explants with defined cpDNA types and a distinguishing probe. The explants obtained showed a bias toward type A cpDNA during tissue culture. The data suggest that chloroplasts with different DNAs in a common nuclear background can multiply at different rates.

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Accession: 002416779

PMID: 24193384

DOI: 10.1007/BF00223809

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