+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Investigation of cryoprotectant toxicity to porcine embryos

, : Investigation of cryoprotectant toxicity to porcine embryos. Theriogenology 41(6): 1291-1298

The objective of this study was to examine the potential toxicity of sucrose (Experiment 1) and of various cryoprotectants (Experiment 2) to porcine preimplantation embryos. In Experiment 1, 65 embryos, ranging from compact morulae to hatched blastocysts, were allocated within donor female across 5 concentrations of sucrose (0, 0.25, 0.50, 1.0, 2.0 M) to determine the highest concentration that would not inhibit subsequent embryo development. After a 48-h post-treatment culture period, the embryos were stained and cell nuclei were counted. The concentration of sucrose affected embryo development (P lt 0.001) and embryo quality (P lt 0.001). Embryos placed into 2.0 M sucrose exhibited poorer development and quality than embryos at the lower 4 concentrations, which were not different from one another. In Experiment 2, 182 embryos of the same developmental stages as in Experiment 1 were collected from 16 donors. Embryos were allotted within donor female to 2 of the 5 concentrations (10, 20, 30, 40, or 50%) of each of 3 cryoprotectants (ethylene glycol, propylene glycol, glycerol). After a 30-sec exposure to a cryoprotectant, the embryos were cultured and stained as in Experiment 1. As the concentration of an individual cryoprotectant increased beyond 30%, embryo development decreased. Embryos exposed to glycerol or propylene glycol exhibited poorer development than did embryos placed into ethylene glycol, especially at concentrations of 40% or higher.

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002416784

PMID: 16727483

DOI: 10.1016/0093-691x(94)90487-4

Download PDF Full Text: Investigation of cryoprotectant toxicity to porcine embryos

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Domer, F.R., 1973: Drug inhibition of PAH transport from cerobrospinal fluid in rabbits. Experimental Neurology 40(2): 414-423

Poddubnyĭ, M.V.; Zeiss, H., 1998: Professor H. Zeiss (1888-1949) and the history of medicine in Russia (70th anniversary of one publication). Problemy Sotsial'noi Gigieny i Istoriia Meditsiny: 60-61

Von Maur, K.; Nelson, E.W.; Holsinger, J.W.; Eliot, R.S., 1972: Hypersensitive carotid sinus syncope treated by implantable demand cardiac pacemaker. American Journal of Cardiology 29(1): 109-110

Daniel, E.; Gangaputra, S.; Kempen, J.H.; Jabs, D.A., 2006: Recurrent nodular scleritis preceding an adult TINU syndrome. To describe the occurrence of nodular scleritis in a patient developing tubulointerstitial nephritis and uveitis (TINU) syndrome. Observational case report. The case notes of a 57-year-old female presenting with recurrent nodular scleritis, who la...

Benvenuti, L.; Cavalli, R., 1994: Energy conservation in artificial drying of round bales. Use of a diesel engine with a heat recovery system, together with a solar collector, was assessed for use in drying of large round bales of lucerne. Drying was carried out in a prefabricated concrete structure with a solar collector on the pitched...

Hitchcock, W. B. Brown, R. F., 1937: Mannum notes, from 12th to 16th May, 1936. South Australian Ornithologist, 14: 20-23

Sharma, S.D.; Fischer, R.; Schoennagel, B.P.; Nielsen, P.; Kooijman, H.; Yamamura, J.; Adam, G.; Bannas, P.; Hernando, D.; Reeder, S.B., 2016: MRI-based quantitative susceptibility mapping (QSM) and R2* mapping of liver iron overload: Comparison with SQUID-based biomagnetic liver susceptometry. We aimed to determine the agreement between quantitative susceptibility mapping (QSM)-based biomagnetic liver susceptometry (BLS) and confounder-corrected R2* mapping with superconducting quantum interference device (SQUID)-based biomagnetic liver...

Rodrigues, S.C.; Pantaleão, L.C.; Nogueira, T.C.; Gomes, Pícia.R.; Albuquerque, G.G.; Nachbar, R.T.; Torres-Leal, F.L.; Caperuto, L.C.; Lellis-Santos, C.; Anhê, G.F.; Bordin, S., 2014: Selective regulation of hepatic lipid metabolism by the AMP-activated protein kinase pathway in late-pregnant rats. The liver plays an essential role in maternal metabolic adaptation during late pregnancy. With regard to lipid metabolism, increased secretion of very low-density lipoprotein (VLDL) is characteristic of late pregnancy. Despite this well-described...

Welman, M.; Lemay, G.; Cohen, E., A., 2007: Role of envelope processing and gp41 membrane spanning domain in the formation of human immunodeficiency virus type I (HIV-1) fusion-competent envelope glycoprotein complex. Human immunodeficiency virus type I (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The...

Pan, I.C.; Whyard, T.C.; Hess, W.R.; Yuasa, N.; Shimizu, M., 1988: Epitopic diversity of African swine fever virus. African swine fever (ASF) is caused by an icosahedral cytoplasmic, double-stranded DNA virus. In the acute form of the disease, pigs die from disseminated intravascular coagulation (DIC) with extensive damage of the free and fixed macrophage syste...