+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Investigation of cryoprotectant toxicity to porcine embryos

Theriogenology 41(6): 1291-1298

Investigation of cryoprotectant toxicity to porcine embryos

The objective of this study was to examine the potential toxicity of sucrose (Experiment 1) and of various cryoprotectants (Experiment 2) to porcine preimplantation embryos. In Experiment 1, 65 embryos, ranging from compact morulae to hatched blastocysts, were allocated within donor female across 5 concentrations of sucrose (0, 0.25, 0.50, 1.0, 2.0 M) to determine the highest concentration that would not inhibit subsequent embryo development. After a 48-h post-treatment culture period, the embryos were stained and cell nuclei were counted. The concentration of sucrose affected embryo development (P lt 0.001) and embryo quality (P lt 0.001). Embryos placed into 2.0 M sucrose exhibited poorer development and quality than embryos at the lower 4 concentrations, which were not different from one another. In Experiment 2, 182 embryos of the same developmental stages as in Experiment 1 were collected from 16 donors. Embryos were allotted within donor female to 2 of the 5 concentrations (10, 20, 30, 40, or 50%) of each of 3 cryoprotectants (ethylene glycol, propylene glycol, glycerol). After a 30-sec exposure to a cryoprotectant, the embryos were cultured and stained as in Experiment 1. As the concentration of an individual cryoprotectant increased beyond 30%, embryo development decreased. Embryos exposed to glycerol or propylene glycol exhibited poorer development than did embryos placed into ethylene glycol, especially at concentrations of 40% or higher.

Accession: 002416784

PMID: 16727483

DOI: 10.1016/0093-691x(94)90487-4

Download PDF Full Text: Investigation of cryoprotectant toxicity to porcine embryos

Related references

Cryoprotectant toxicity in penaeid prawn embryos. Cryobiology. 33(1): 172-177, 1996

Cryoprotectant agent toxicity in porcine articular chondrocytes. Cryobiology 61(3): 297-302, 2011

Cryoprotectant toxicity and permeability studies of zebrafish embryos. Cryobiology 30(6): 640, 1993

Cryoprotectant toxicity and cryoprotectant toxicity reduction in search of molecular mechanisms. Cryobiology 26(6): 537, 1989

Cryoprotectant toxicity and cryopreservation success in IVF-Derived domestic cat embryos after embryo transfer. Theriogenology 51(1): 174, Jan 1, 1999

Effects of cryoprotectant toxicity on the embryos and larvae of pacific white shrimp Litopenaeus vannamei. Aquaculture 20 242(1-4): 655-670, 2004

Cryoprotectant microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos. Aquaculture 261(3): 897-903, 2006

Effects of a complex cryoprotectant on the cryopreservation rates of embryos fertilized in vitro with special reference to the addition of plant cryoprotectant factors. Uber den Einfluss eines komplexen Einfrierbasismediums auf die Ergebnisse der Kryokonservierung von IVF Embryonen unter besonderer Berucksichtigung des Zusatzes eines pflanzlichen Gefrierschutzfaktors: 96 pp., 1993

Effects of cryoprotectant toxicity and temperature sensitivity on the embryos of black tiger shrimp (Penaeus monodon). Aquaculture 246(1/4): 275-284, 2005

Effects of cryoprotectant toxicity on embryos of the chinese mitten crab, eriocheir sinensis decapoda, brachyura. Crustaceana (Leiden) 84(3): 281-291, 2011