+ Translate

Investigation of cryoprotectant toxicity to porcine embryos

, : Investigation of cryoprotectant toxicity to porcine embryos. Theriogenology 41(6): 1291-1298

The objective of this study was to examine the potential toxicity of sucrose (Experiment 1) and of various cryoprotectants (Experiment 2) to porcine preimplantation embryos. In Experiment 1, 65 embryos, ranging from compact morulae to hatched blastocysts, were allocated within donor female across 5 concentrations of sucrose (0, 0.25, 0.50, 1.0, 2.0 M) to determine the highest concentration that would not inhibit subsequent embryo development. After a 48-h post-treatment culture period, the embryos were stained and cell nuclei were counted. The concentration of sucrose affected embryo development (P lt 0.001) and embryo quality (P lt 0.001). Embryos placed into 2.0 M sucrose exhibited poorer development and quality than embryos at the lower 4 concentrations, which were not different from one another. In Experiment 2, 182 embryos of the same developmental stages as in Experiment 1 were collected from 16 donors. Embryos were allotted within donor female to 2 of the 5 concentrations (10, 20, 30, 40, or 50%) of each of 3 cryoprotectants (ethylene glycol, propylene glycol, glycerol). After a 30-sec exposure to a cryoprotectant, the embryos were cultured and stained as in Experiment 1. As the concentration of an individual cryoprotectant increased beyond 30%, embryo development decreased. Embryos exposed to glycerol or propylene glycol exhibited poorer development than did embryos placed into ethylene glycol, especially at concentrations of 40% or higher.

Accession: 002416784

PMID: 16727483

DOI: 10.1016/0093-691x(94)90487-4

Download PDF Full Text: Investigation of cryoprotectant toxicity to porcine embryos

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Newton, S.S.muel; Subramoniam, T., 1996: Cryoprotectant toxicity in penaeid prawn embryos. Cryopreservation of penaeid prawn embryos has definite applications in the aquaculture industry. It can be considered a viable alternative to solve the "seed" scarcity faced during the "lean" season. Presently, there is no prot...

Jomha, N.M.; Weiss, A.D.H.; Fraser Forbes, J.; Law, G.K.; Elliott, J.A.W.; McGann, L.E., 2011: Cryoprotectant agent toxicity in porcine articular chondrocytes. Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprote...

Zhang, T.; Rawson, D.M., 1993: Cryoprotectant toxicity and permeability studies of zebrafish embryos. Cryobiology 30(6): 640

Fahy G.M.; Lilley T.H.; Lindell H.; Douglas M.S.; Meryman H.T., 1989: Cryoprotectant toxicity and cryoprotectant toxicity reduction in search of molecular mechanisms. Cryobiology 26(6): 537

Swanson, W.F.; Mcrae, M.A.; Wildt, D.E.; Rall, W.F., 1999: Cryoprotectant toxicity and cryopreservation success in IVF-Derived domestic cat embryos after embryo transfer. Theriogenology 51(1): 174, Jan 1

Dong, Q.L.n, J.H.ang, C., 2004: Effects of cryoprotectant toxicity on the embryos and larvae of pacific white shrimp Litopenaeus vannamei. We tested the toxicity of four cryoprotectants (methanol, ethylene glycol, propylene glycol and dimethyl sulfoxide (DMSO) on embryos and larvae of the Pacific white shrimp Litopenaeus vannamei. The developmental stages tested include prenauplius e...

Beirao, J.; Robles, V.; Herraez, M.P.; Sarasquete, C.; Dinis, M.T.; Cabrita, E., 2006: Cryoprotectant microinjection toxicity and chilling sensitivity in gilthead seabream (Sparus aurata) embryos. Cryopreservation of fish embryos requires an optimal distribution of cryoprotectants inside all embryo compartments. Traditional techniques for the incorporation of cryoprotectants (CPAs) have failed to protect all fish compartments, especially t...

Walter, C., 1993: Effects of a complex cryoprotectant on the cryopreservation rates of embryos fertilized in vitro with special reference to the addition of plant cryoprotectant factors. Bovine embryos, frozen after in vitro culture for 7 days, had a higher thawing rate than those cultured for 6 days, and a lower percentage of degenerated embryos, the differences being significant for blastocysts cultured for 24 and 72 h. Use of a...

Vuthiphandchai, V.; Pengpun, B.; Nimrat, S., 2005: Effects of cryoprotectant toxicity and temperature sensitivity on the embryos of black tiger shrimp (Penaeus monodon). The response of black tiger shrimp (Penaeus monodon) embryos in terms of hatching success to cryoprotectants and temperature sensitivity was investigated for basic knowledge to enhance cryopreservation success of P monodon embryos. Three stages of...

Huang, X.; Zhuang, P.; Zhang, L.; Yao, Z.; Liu, T.; Liu, J.; Feng, G., 2011: Effects of cryoprotectant toxicity on embryos of the chinese mitten crab, eriocheir sinensis decapoda, brachyura. The response of embryos of the Chinese mitten crab (Eriocheir sinensis) in terms of survival to cryoprotectants was investigated for basic knowledge to enhance cryopreservation success of E. sinensis embryos. Five stages of embryonic development (...