EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Investigation of cryoprotectant toxicity to porcine embryos


, : Investigation of cryoprotectant toxicity to porcine embryos. Theriogenology 41(6): 1291-1298

The objective of this study was to examine the potential toxicity of sucrose (Experiment 1) and of various cryoprotectants (Experiment 2) to porcine preimplantation embryos. In Experiment 1, 65 embryos, ranging from compact morulae to hatched blastocysts, were allocated within donor female across 5 concentrations of sucrose (0, 0.25, 0.50, 1.0, 2.0 M) to determine the highest concentration that would not inhibit subsequent embryo development. After a 48-h post-treatment culture period, the embryos were stained and cell nuclei were counted. The concentration of sucrose affected embryo development (P lt 0.001) and embryo quality (P lt 0.001). Embryos placed into 2.0 M sucrose exhibited poorer development and quality than embryos at the lower 4 concentrations, which were not different from one another. In Experiment 2, 182 embryos of the same developmental stages as in Experiment 1 were collected from 16 donors. Embryos were allotted within donor female to 2 of the 5 concentrations (10, 20, 30, 40, or 50%) of each of 3 cryoprotectants (ethylene glycol, propylene glycol, glycerol). After a 30-sec exposure to a cryoprotectant, the embryos were cultured and stained as in Experiment 1. As the concentration of an individual cryoprotectant increased beyond 30%, embryo development decreased. Embryos exposed to glycerol or propylene glycol exhibited poorer development than did embryos placed into ethylene glycol, especially at concentrations of 40% or higher.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002416784

PMID: 16727483

DOI: 10.1016/0093-691x(94)90487-4

PDF Full Text: Investigation of cryoprotectant toxicity to porcine embryos


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Clarke, M.; Cai, G.; Saleh, S.; Buller, K.M.; Spencer, S.J., 2014: Being suckled in a large litter mitigates the effects of early-life stress on hypothalamic-pituitary-adrenal axis function in the male rat. The perinatal environment influences stress responses in the long-term, as does body composition. Male rats suckled in large litters, where they have reduced access to milk and attention from the dam, are less anxious and have attenuated hypothala...

Nacimiento A.C.; Adler G.; Nieschalk M., 1987: Neurophysiological analysis of somatosensory evoked potentials sep by means of input ouput relationships. Pfluegers Archiv European Journal of Physiology 408(SUPPL 1): R61

Whitford, Re, 1995: Inexpensive ways to increase your gross--right now!. Proceedings of the North American Veterinary Conference(9): 626-627

Rubinstein, M., 1939: Action de lhypoglycemie et de lhyperglycemie provoquees sur la chronaxie vestibulaire. 1-5 units of insulin subcut. injected into [male] or [female] rabbits (1.9-3.5 kg) increased vestibular "excitability" as expressed by falling of the 3 chronaxias indicated in a preceding paper. Reduction of these chronaxias runs paralle...

Aked, L., 1994: Tackling tractor trouble. Results of research at the Swedish Institute of Agricultural Engineering into the causes of tractor breakdown are outlined. These were considered to be due to 1 of 7 reasons: normal wear and tear; design errors and wrong materials; careless mainte...

Samson, Willis K., 1997: Cardiovascular hormones. Conn, P M [Editor], Melmed, S [Editor] Endocrinology: Basic and clinical principles : 361-376

Levin, V.A.; Hess, K.R., 2016: Study design considerations for phase II trials of anaplastic gliomas and its impact on phase III survival studies. NlmCategory="UNASSIGNED">1501 Background: Phase 2 trials for anaplastic gliomas (AG; WHO 3) such as anaplastic astrocytoma, oligoastrocytoma, and oligodendroglioma remain the most productive path for the development of new therapeutic...

Thal, J.; Steffen, M.; Meier, B.; Schneider, E.; Adriany, A.; Usleber, E., 2011: Development of an enzyme immunoassay for the antibiotic cefquinome and its application for residue determination in cow's milk after therapeutical mastitis treatment. The aim of this study was to develop and evaluate an enzyme immunoassay (EIA) for the cephalosporin antibiotic in milk, in combination with a new microbiological test system (brilliant black reduction test, BRT-P). Polyclonal antibodies against ce...

Finckh, A., 2014: Comparative effectiveness of biologic antirheumatic therapies in rheumatoid arthritis after failure to respond to a first TNF inhibitor. Evaluation of: Gomez-Reino JJ, Maneiro JR, Ruiz J et al. Comparative effectiveness of switching to alternative tumour necrosis factor (TNF) antagonists versus switching to rituximab in patients with rheumatoid arthritis who failed previous TNF ant...

Klonaris, C.; Lioudaki, S.; Katsargyris, A.; Psathas, E.; Kouvelos, G.; Doulaptsis, M.; Verikokos, C.; Kouraklis, G., 2014: Late open conversion after failed endovascular aortic aneurysm repair. Endovascular aortic aneurysm repair (EVAR) is widely used for the treatment of abdominal aortic aneurysms. Complications secondary to EVAR are also treated with endovascular techniques. When this is not applicable, open surgical repair is mandator...