EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Investigation of subpopulation heterogeneity and plasmid stability in recombinant escherichia coli via a simple segregated model


, : Investigation of subpopulation heterogeneity and plasmid stability in recombinant escherichia coli via a simple segregated model. Biotechnology and Bioengineering 42(2): 222-234

Many microbial and cell cultures exhibit phenomena that can best be described using a segregated modeling approach. Heterogeneities are more marked in recombinant cell cultures because subpopulations, which often exhibit different growth and productivity characteristics, are more easily identified by selective markers. A simple segregated mathematical model that simulates the growth of recombinant Escherichia coli cells is developed. Subpopulations of different growth rate, plasmid replication rate, and plasmid segregation probability are explicitly considered. Results indicate that a third mechanism of plasmid instability, referred to here as a "downward selective pressure," is significant when describing plasmid loss in batch and chemostat cultures. Also, the model agrees well with experimental data from cultures under antibiotic selective pressure. Finally, model simulations of chemostat cultures reveal the importance of initial conditions on culture stability and the possible presence of nonrandom partitioning functions.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002416884

PMID: 18612983

DOI: 10.1002/bit.260420210

Download PDF Full Text: Investigation of subpopulation heterogeneity and plasmid stability in recombinant escherichia coli via a simple segregated model


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Cortemiglia, G.C.; Manfredini, U., 2003: Climatic dynamics of the thermometric (1861div2002) and pluviometric (1812div2002) series of Pavia (Lombardy, Italy). The two historical series of Pavia, relevant to the observational data of the mean monthly temperatures in the time interval 1861div2002 and of the monthly precipitations in the time interval 1812div2002, have been analyzed with a statistical appr...

Callisaya, M.L.; Blizzard, C.L.; Wood, A.G.; Thrift, A.G.; Wardill, T.; Srikanth, V.K., 2016: Longitudinal Relationships Between Cognitive Decline and Gait Slowing: The Tasmanian Study of Cognition and Gait. Gait slowing and cognitive decline are both common in older people. Although cross-sectionally related, the longitudinal associations between specific cognitive functions and gait speed are less well understood. We aimed to determine whether decli...

Nielsen, H.K.; Lybecker, H., 1991: Lipiduria after intralipid infusion of a lipid emulsion in a boy with an abdominal trauma. A 4-year-old boy with an abdominal trauma had lipiduria following treatment with total intravenous nutrition for 4 days. Renal function was normal throughout the course, and the lipiduria ceased after withdrawal of the intravenous nutrition. The l...

Jpn Soc Plast Reconstr Surg, 1975: The japanese society for plastic and reconstructive surgery the 3rd mokuriku area meeting of the japanese society for plastic and reconstructive surgery kanazawa japan march 3 1974. Japanese Journal of Plastic & Reconstructive Surgery 18(4): 432-436

Jonsson, K.B., 2005: The role of fibroblast growth factor 23 in renal disease. Warthin tumours (WT) and malignant lymphomas are only rarely associated, and most are examples of involvement of the lymphoid stroma of WT by a disseminated lymphoma. This report describes a case where excision of a parotid mass led to the initial...

McClean, Sán., 2016: Prospects for subunit vaccines: Technology advances resulting in efficacious antigens requires matching advances in early clinical trial investment. NlmCategory="UNASSIGNED">With the continued march of antimicrobial resistance, a renewed impetus for better vaccines has been heralded. Identification of potent subunit vaccines has been greatly facilitated by recent developments in r...

Lorenzo, G.A.; Estepa, A.; Chilmonczyk, S.; Coll, J.M., 1995: Different peptides from hemorrhagic septicemia rhabdoviral proteins stimulate leukocyte proliferation with individual fish variation. Trout leucocytes from most of the survivors of viral hemorrhagic septicemia virus (VHSV) infections were capable of in vitro proliferation (T-like response) when cultured in the presence of short synthetic peptides designed from the G and the N cD...

Covasa, M.; Ritter, R.C.; Burns, G.A., 1999: NMDA receptor blockade accelerates gastric emptying. Amino Acids (Vienna) 17(1): 39

Guseinov V.A.; Runov V.I., 1973: Strain differences of the structure of verticillium dahliae dna. Doklady Akademii Nauk SSSR Seriya Biologiya 212(3): 764-766

Zaporozhtsev, L.N.; Arzumanian, S.O.; Serov, V.N., 1980: Classification of devices for cultivating microorganisms. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii: 44-47