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Investigation of the the black widow spider neurotoxin receptor. I. Characteristics of the membrane-bound and solubilized receptor from bovine brain


, : Investigation of the the black widow spider neurotoxin receptor. I. Characteristics of the membrane-bound and solubilized receptor from bovine brain. Bioorganicheskaya Khimiya 16(2): 149-157

alpha -latrotoxin from the venom of Latrodectus mactans tredecimguttatus labelled with I125, was specifically bound by a bovine brain cortex membrane receptor and formed a stable, slowly dissociating complex with Kd = 1.6.10-10 and Bmax = 0.5 pmol/mg protein. Reversible dissociation of the membrane-bound toxin occurred in solutions containing at least 2 M NaCl or KCl and pH of 9.5. Polyclonal anti-toxin antibodies did not noticeably increase the speed of dissociation.

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