Section 3
Chapter 2,420

Isolation of different non-cytopathogenic bovine viral diarrhoea (BVD) viruses from cytopathogenic BVD virus stocks using reverse plaque formation method

Nakamura, S.; Fukusho, A.; Inoue, Y.; Sasaki, H.; Ogawa, N.

Veterinary Microbiology 38(1-2): 173-179


ISSN/ISBN: 0378-1135
PMID: 8128599
DOI: 10.1016/0378-1135(93)90084-k
Accession: 002419157

Non-cytopathogenic (NCP) bovine viral diarrhoea (BVD) viruses were isolated from three cytopathogenic (CP) BVD virus stocks using the reverse plaque formation method, which was based on intrinsic interference. By means of an exaltation of Newcastle disease virus (END) test, these NCP BVD viruses were divided into two groups; END phenomenon positive (END+) and END phenomenon negative (END-) viruses. Additionally, the END+ NCP BVD viruses interfered only with CP BVD virus whereas the END- NCP BVD viruses interfered with vesicular stomatitis virus as well as CP BVD virus. Differences in antigenicity existed among the three CP strains, however, each group of parent CP BVD virus and derivative NCP BVD virus was antigenically indistinguishable.

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