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Monoclonal antibodies specific for Laelius pedatus (Bethylidae) and Bracon hebetor (Braconidae), two hymenopteran parasitoids of stored-products pests


Monoclonal antibodies specific for Laelius pedatus (Bethylidae) and Bracon hebetor (Braconidae), two hymenopteran parasitoids of stored-products pests



Biological Control 1(4): 302-308



ISSN/ISBN: 1049-9644

Monoclonal antibodies (MAbs) generated against Laelius pedatus and Bracon hebetor reacted with all life stages and both sexes. In Western blots of acrylamide and agarose gels, the MAbs recognized high molecular weight proteins (> 500 KDa) composed of multiple subunits, with mildly acidic-neutral isoelectric focusing points. The MAbs did not crossreact with the additional 22 insect species studied in an indirect enzyme-linked immunoabsorbent assay.

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