Morphogenesis in the culture of mesophyll protoplasts of pea (Pisum sativum L.)

Zubko, E.I.; Kuchuk, N.V.

Sovremennye metody i podkhody v selektsii rastenii: 51-55


Accession: 002437131

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A methodology for increasing the percentage of viable protoplasts obtained was developed, and a suitable nutrient medium composition was established for maintaining viable protoplast-derived clones for 6 months. In some genotypes, shoot morphogenesis was noted at a low frequency. Optimum conditions for plant regeneration from cell clones were determined, and the regeneration efficiency was increased.